The experimental trial was conducted to assess the effects of dietary pyridoxine on growth performance, physiological metabolics, intestinal enzymatic activities and antioxidant properties of Pelteobagrus fulvidraco. Six isonitrogenous and isolipidic diets (420 g/kg protein and 70 g/kg lipid) were formulated to contain 3.69 (control), 5.45, 7.31,11.17, 19.43 and 33.94 mg/kg pyridoxine. Four groups of 30 fish (3.85 ± 0.01 g) per tank (300-L cylindrical fiberglass tanks) for each diet were fed to apparent satiation twice daily for 63 days. Blood, intestine and hepatopancreas samples were obtained for metabolic, enzymatic activities and antioxidant properties analyses. The results showed that significantly higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER), protein retention efficiency (PRE), whole-body crude protein, intestinal protease, alkaline phosphatase, Na^+/K⁺-ATPase, γ-glutamyl transpeptidase and significantly lower feed conversion ratio were observed in fish fed diet with 7.31 mg/kg compared to those in control group (P < 0.05). There was a significant increase of intestinal villus length, villus width and muscular thickness in fish fed 5.45 or 7.31 mg/kg pyridoxine diets compared with those in control group (P < 0.05). The fish fed with 7.31 or 11.17 mg/kg pyridoxine diets had significantly higher intestosomatic index, relative intestinal length, serum total protein, triglyceride, cholesterol, hepatic glutathione peroxidase, superoxide dismutase (SOD), aspartate aminotransferase, alanine aminotransferase and lower viscerasomatic index, serum glucose, hepatic malondialdehyde compared to those in control group (P < 0.05). Based on WG, FCR, PER, crude protein, protease and SOD activities, the optimal dietary pyridoxine requirement of yellow catfish was estimated to be 6.70 −7.42 mg/kg using broken-line regression analysis.

该实验旨在评估膳食吡哆醇对黄颡鱼生长性能、生理代谢、肠道酶活性和抗氧化特性的影响. 六种等氮和等脂饮食（420 g/kg 蛋白质和 70 g/kg 脂质）配制为含有 3.69（对照）、5.45、7.31、11.17、19.43 和 33.94 mg/kg 吡哆醇。四组 30 条鱼 (3.85 ± 0.01 g) 每个鱼缸（300-L 圆柱形玻璃纤维鱼缸），每天两次喂食至明显饱食，共 63 天。获得血液、肠和肝胰腺样本用于代谢、酶活性和抗氧化特性分析。结果表明，显着提高增重（WG）、比生长率（SGR）、蛋白质效率比（PER）、蛋白质保留效率（PRE）、全身粗蛋白、肠道蛋白酶、碱性磷酸酶、Na ^+/ K ⁺7.31 mg/kg的鱼饲料与对照组相比，观察到-ATP酶、γ-谷氨酰转肽酶和饲料转化率显着降低（P  < 0.05）。与对照组相比，饲喂5.45或7.31 mg/kg吡哆醇的鱼肠绒毛长度、绒毛宽度和肌肉厚度均显着增加（P  < 0.05）。喂食 7.31 或 11.17 mg/kg 吡哆醇日粮的鱼的肠体指数、相对肠长、血清总蛋白、甘油三酯、胆固醇、肝谷胱甘肽过氧化物酶、超氧化物歧化酶 (SOD)、天冬氨酸氨基转移酶、丙氨酸氨基转移酶和较低的内脏指数显着升高，血糖、肝丙二醛与对照组比较（P < 0.05)。根据 WG、FCR、PER、粗蛋白、蛋白酶和 SOD 活性，使用折线回归分析估计黄鲶鱼的最佳日粮吡哆醇需求量为 6.70 -7.42 mg/kg。