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Shape-based separation of drug-treated Escherichia coli using viscoelastic microfluidics
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-05-09 , DOI: 10.1039/d2lc00339b
Tianlong Zhang 1, 2 , Hangrui Liu 1 , Kazunori Okano 2 , Tao Tang 2 , Kazuki Inoue 2 , Yoichi Yamazaki 2 , Hironari Kamikubo 2 , Amy K Cain 3 , Yo Tanaka 4 , David W Inglis 1 , Yoichiroh Hosokawa 2 , Yalikun Yaxiaer 2 , Ming Li 1, 5
Affiliation  

Here, we achieve shape-based separation of drug-treated Escherichia coli (E. coli) by viscoelastic microfluidics. Since shape is critical for modulating biological functions of E. coli, the ability to prepare homogeneous E. coli populations adopting uniform shape or sort bacterial sub-population based on their shape has significant implications for a broad range of biological, biomedical and environmental applications. A proportion of E. coli treated with 1 μg mL−1 of the antibiotic mecillinam were found to exhibit changes in shape from rod to sphere, and the heterogeneous E. coli populations after drug treatment with various aspect ratios (ARs) ranging from 1.0 to 5.5 were used for experiment. We demonstrate that E. coli with a lower AR, i.e., spherical E. coli (AR ≤ 1.5), are directed toward the middle outlet, while rod-shaped E. coli with a higher AR (AR > 1.5) are driven to the side outlets. Further, we demonstrate that the separation performance of the viscoelastic microfluidic device is influenced by two main factors: sheath-to-sample flow rate ratio and the concentration of poly-ethylene-oxide (PEO). To the best of our knowledge, this is the first report on shape-based separation of a single species of cells smaller than 4 μm by microfluidics.

中文翻译:

使用粘弹性微流体基于形状分离药物处理的大肠杆菌

在这里,我们通过粘弹性微流体实现了药物处理的大肠杆菌大肠杆菌)的基于形状的分离。由于形状对于调节大肠杆菌的生物学功能至关重要,因此能够制备采用统一形状的同质大肠杆菌种群或根据其形状对细菌亚群进行分类的能力对广泛的生物、生物医学和环境应用具有重要意义。发现用 1 μg mL -1抗生素 mecillinam处理的一部分大肠杆菌表现出从棒状到球状的形状变化,而异质大肠杆菌以1.0至5.5范围内的各种纵横比(AR)进行药物治疗后的人群用于实验。我们证明了具有较低 AR 的大肠杆菌,球形大肠杆菌(AR ≤ 1.5),被导向中间出口,而具有较高 AR (AR > 1.5) 的杆状大肠杆菌被驱赶到中间出口。侧插座。此外,我们证明了粘弹性微流体装置的分离性能受两个主要因素的影响:鞘液与样品的流速比和聚环氧乙烷 (PEO) 的浓度。据我们所知,这是第一份关于通过微流体技术对小于 4 μm 的单一种类细胞进行基于形状分离的报告。
更新日期:2022-05-09
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