Potato virus X (PVX), a species of the genus Potexvirus, is a plant pathogenic virus that causes severe symptoms such as mild mosaic, crinkling, necrosis, and mottling on leaves. The objectives of the present study were to investigate the effect of PVX virus infection on the metabolic system in nontransgenic and Arabidopsis thaliana production of anthocyanin pigment 1 (AtPAP1) transgenic tobacco using transcript expression analysis and metabolic profiling. Potato virus X inoculation increased the gene expression of phenylpropanoid and flavonoid biosynthesis and the production of chlorogenic acid, p-coumaric acid, benzoic acid, rutin, quercetin, and kaempferol in nontransgenic tobacco leaves. However, in the AtPAP1 transgenic tobacco leaves, PVX inoculation decreased the expression of AtPAP1 and phenylpropanoid and flavonoid biosynthesis genes, and the production of phenolics and anthocyanin also declined. In contrast, the levels of amino acids and tricarboxylic acid (TCA) cycle intermediates increased after infection in the AtPAP1 transgenic plant leaves. To date, these results have not been reported previously. We suggest that PVX infection decreases AtPAP1 expression, leading to the downregulation of phenylpropanoid and flavonoid biosynthesis in transgenic plants.

中文翻译：

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感染马铃薯病毒 X (PVX) 的非转基因和 AtPAP1 转基因烟草的基因表达和代谢分析

马铃薯病毒 X (PVX) 是Potexvirus属的一种，是一种植物病原病毒，可引起严重的症状，如叶片轻度花叶、皱缩、坏死和斑点。本研究的目的是使用转录表达分析和代谢谱分析研究 PVX 病毒感染对非转基因和拟南芥生产花青素色素 1 ( AtPAP 1) 转基因烟草代谢系统的影响。马铃薯病毒X接种增加了苯丙素和黄酮生物合成的基因表达和绿原酸的产生，p-非转基因烟叶中的香豆酸、苯甲酸、芦丁、槲皮素和山奈酚。然而，在AtPAP 1 转基因烟叶中，接种PVX 降低了AtPAP 1 和苯丙素和黄酮类生物合成基因的表达，酚类物质和花青素的产生也下降。相比之下， AtPAP 1 转基因植物叶片感染后氨基酸和三羧酸 (TCA) 循环中间体的水平增加。迄今为止，这些结果以前没有被报道过。我们认为 PVX 感染会降低AtPAP 1 的表达，从而导致转基因植物中苯丙烷和类黄酮生物合成的下调。