A facile visualized solid-phase detection of virus-specific nucleic acid sequences through an upconversion activated linear luminescence recovery process,Analyst

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A facile visualized solid-phase detection of virus-specific nucleic acid sequences through an upconversion activated linear luminescence recovery process
Analyst ( IF 3.6 ) Pub Date : 2022-05-07 , DOI: 10.1039/d2an00382a
Xiaorong Liu ₁ , Chaonan He ₂ , Qi Huang ₁ , Mengmeng Yu ₃ , Zhuang Qiu ₃ , Haoxin Cheng ₁ , Yifei Yang ₃ , Xian Hao ₃ , Xiaolei Wang _{1,

2}

Affiliation

The development of portable solid-phase biosensors is of great significance for point-of-care testing (POCT). In this work, we constructed a simple visualized solid-phase biosensor based on luminescence resonance energy transfer (LRET) from upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) for quantitative detection of virus-specific nucleic acid sequences. The detection data showed that there was a linear relationship between the luminescence recovery of UCNPs and the concentrations of the target within the range of 5–100 nM, and the limit of detection (LOD) was 0.326 nM. Additionally, the luminescence recovery of UCNPs was visualized and quantitatively analyzed using a home-built luminescence image capture device and an open-source ImageJ software that can analyze and process images. Compared with conventional liquid-phase biosensors, the solid-phase analysis method we constructed not only has advantages in cost, portability and stability, but also is more conducive to the rapid acquisition and storage of the detected sample, which is expected to become a fast, efficient and reliable detection platform for POCT.

中文翻译：

通过上转换激活的线性发光恢复过程轻松可视化固相检测病毒特异性核酸序列

便携式固相生物传感器的开发对于即时检测（POCT）具有重要意义。在这项工作中，我们构建了一个简单的可视化固相生物传感器，该传感器基于从上转换纳米粒子 (UCNPs) 到金纳米粒子 (AuNPs) 的发光共振能量转移 (LRET)，用于定量检测病毒特异性核酸序列。检测数据显示，UCNPs的发光恢复率与靶标浓度在5~100 nM范围内呈线性关系，检测限（LOD）为0.326 nM。此外，使用自制的发光图像捕获设备和可以分析和处理图像的开源 ImageJ 软件对 UCNPs 的发光恢复进行可视化和定量分析。

更新日期：2022-05-11

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