Human IgG is one of the most important immunoglobulins in the human body. The present study described the fabrication of four kinds of layer-by-layer structures of copper metal-organic frameworks (Cu-MOFs) on the working electrode by electrodeposition, which were then applied as an electrochemical sensor for the sensitive determination of IgG in serum. First, MOFs synthesized using different deposition potentials are expected to have varied morphology and properties. Herein, four copper MOFs (Cu-MOFs) were electrosynthesized by a simple and direct reduction approach. The as-synthesized Cu-MOFs exhibit varied morphology and electrocatalytic behavior. Then, IgG was employed as a template in the electropolymerization of pyrrole-imprinted films on the surface of glassy carbon electrodes. Finally, the template protein was removed to form a molecularly imprinted film with the capability to qualitatively and quantitatively signaling of IgG. Under optimized conditions, the sensor for IgG exhibits a wide detection range of 0.01–10 ng mL⁻¹ with a limit of detection (LOD) of 3 pg mL⁻¹ (S/N = 3). Besides, other parameters including the selectivity, reproducibility (RSD 3.6%), and recovery rate (95.2–102.0%) are all satisfactory. The practicability of the sensor was verified by detecting IgG in human serum samples, which indicated that the sensor was suitable for potential clinical applications.

人IgG是人体内最重要的免疫球蛋白之一。本研究描述了通过电沉积在工作电极上制备四种逐层结构的铜金属有机骨架 (Cu-MOFs)，然后将其用作电化学传感器，用于灵敏测定血清中的 IgG。 . 首先，预计使用不同沉积电位合成的 MOF 具有不同的形态和性质。在此，通过简单直接的还原方法电合成了四种铜 MOF（Cu-MOF）。合成的Cu-MOFs表现出不同的形态和电催化行为。然后，IgG 被用作模板在玻碳电极表面上的吡咯印迹薄膜的电聚合中。最后，去除模板蛋白以形成分子印迹膜，具有定性和定量 IgG 信号传导的能力。在优化条件下，用于 IgG 的传感器具有 0.01–10 ng mL 的宽检测范围^-1，检测限 (LOD) 为 3 pg mL ^-1 (S/N = 3)。此外，选择性、重现性 (RSD 3.6%) 和回收率 (95.2–102.0%) 等其他参数均令人满意。通过检测人血清样本中的IgG，验证了该传感器的实用性，表明该传感器适用于潜在的临床应用。