Nuciferine is an important drug candidate for the treatment of many diseases. However, there is no general method for its low - cost production. In this work, a feasible method for the production of nuciferine from lotus leaf, using ultrasonic-assisted extraction-solid phase extraction (UAE-SPE) as extraction and cleanup procedure, was developed. Petroleum ether and silica gel have been successfully used as extraction solvent and adsorbent to integrate UAE with SPE, respectively. Except for filtration, no treatment (e.g. concentration and redissolution, etc) was needed on UAE extract before SPE and the effluents obtained in the loading process of SPE could be used as UAE extraction solvent without purification. No obvious decline in the extraction efficiency of UAE and adsorption capacity of SPE was observed at least for 5 runs, which provides a feasible way for the continuous production of nuciferine in industry, i.e. Cyclic UAE-SPE. Moreover, SPE column could be conveniently regenerated and reused without significant decline in its adsorption capacity at least for 5 cycles, which can be used to reduce the cost of the whole system further. In comparison with other cleanup procedures, Cyclic UAE-SPE showed apparent advantages in energy conservation and emission reduction. LLE and crystallization were applied to separate nuciferine from other impurities further. Under optimum conditions, the total recovery rate of nuciferine with a purity over 90.0% from lotus leaf reached 50.1%. All in all, the developed method has advantages in convenient operation, low cost, and high efficiency, thus, is fitting for the production of high purity nuciferine.

Nuciferine是治疗许多疾病的重要候选药物。但是，它的低成本生产并没有通用的方法。在这项工作中，开发了一种利用超声辅助萃取-固相萃取（UAE-SPE）作为提取和净化程序的荷叶生产荷叶碱的可行方法。石油醚和硅胶分别成功地用作萃取溶剂和吸附剂，将阿联酋与 SPE 相结合。除过滤外，阿拉伯联合酋长国萃取液在SPE前无需进行任何处理（如浓缩和再溶解等），SPE加载过程中得到的流出物无需纯化即可用作阿拉伯联合酋长国的萃取溶剂。至少在 5 次运行中，UAE 的萃取效率和 SPE 的吸附能力没有明显下降，为工业上连续生产nuciferine提供了一条可行的途径，即CyclicUAE-SPE。此外，SPE柱可以方便地再生和重复使用，至少5个循环后其吸附能力不会明显下降，可以进一步降低整个系统的成本。与其他净化工艺相比，CyclicUAE-SPE在节能减排方面具有明显优势。应用 LLE 和结晶进一步从其他杂质中分离出荷叶碱。在最佳条件下，荷叶中纯度在90.0%以上的荷叶碱总回收率达到50.1%。综上所述，该方法具有操作方便、成本低、效率高等优点，适用于高纯度荷叶碱的生产。