BB0761, a MepM homolog, contributes to Borrelia burgdorferi cell division and mammalian infectivity,Molecular Microbiology

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BB0761, a MepM homolog, contributes to Borrelia burgdorferi cell division and mammalian infectivity
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-05-05 , DOI: 10.1111/mmi.14916
Christina Thompson ₁ , Sierra George ₁ , Maria L White ₂ , Prahathees J Eswara ₂ , Zhiming Ouyang ₁

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M23 family endopeptidases play important roles in cell division and separation in a wide variety of bacteria. Recent studies have suggested that these proteins also contribute to bacterial virulence. However, the biological function of M23 peptidases in pathogenic spirochetes remains unexplored. Here, we describe Borrelia burgdorferi, the bacterial pathogen causing Lyme disease, requires a putative M23 family homolog, BB0761, for spirochete morphology and cell division. Indeed, the inactivation of bb0761 led to an aberrant filamentous phenotype as well as the impairment of B. burgdorferi growth in vitro. These phenotypes were complemented not only with B. burgdorferi bb0761, but also with the mepM gene from E. coli. Moreover, the bb0761 mutant showed a complete loss of infectivity in a murine model of Lyme borreliosis. Resistance of the mutant to osmotic and oxidative stresses was markedly reduced. Our combined results indicate that BB0761 contributes to B. burgdorferi cell division and virulence.

中文翻译：

BB0761 是一种 MepM 同系物，有助于伯氏疏螺旋体细胞分裂和哺乳动物感染性

M23 家族内肽酶在多种细菌的细胞分裂和分离中起重要作用。最近的研究表明，这些蛋白质也有助于细菌毒力。然而，M23 肽酶在致病性螺旋体中的生物学功能仍未得到探索。在这里，我们描述了引起莱姆病的细菌病原体伯氏疏螺旋体，它需要一个推定的 M23 家族同系物 BB0761，用于螺旋体形态和细胞分裂。实际上，bb0761的失活会导致异常的丝状表型以及伯氏疏螺旋体体外生长受损。这些表型不仅与B. burgdorferi bb0761 互补，而且与来自大肠杆菌。此外，bb0761突变体在莱姆疏螺旋体病小鼠模型中表现出完全丧失传染性。突变体对渗透压和氧化应激的抵抗力显着降低。我们的综合结果表明 BB0761 有助于伯氏疏螺旋体细胞分裂和毒力。

更新日期：2022-05-05

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