Cellular point-of-care diagnostics using an inexpensive layer-stack microfluidic device,Lab on a Chip

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Cellular point-of-care diagnostics using an inexpensive layer-stack microfluidic device
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-05-06 , DOI: 10.1039/d2lc00162d
Kilean Lucas ₁ , Juhyun Oh ₁ , Jan Hoelzl ₁ , Ralph Weissleder _{1,

2}

Affiliation

Cellular analyses are increasingly used to diagnose diseases at point-of-care and global healthcare settings. Some analyses are simple as they rely on chromogenic stains (blood counts, malaria) but others often require higher multiplexing to define and quantitate cell populations (cancer diagnosis, immunoprofiling). Simplifying the latter with inexpensive solutions represents a current bottleneck in designing start-end pipelines. Based on the hypothesis that novel film adhesives could be used to create inexpensive disposable devices, we tested a number of different designs and materials, to rapidly perform 12–15 channel single-cell imaging. Using an optimized passive pumping layer-stack microfluidic (PLASMIC) device (<1 $ in supplies) we show that rapid, inexpensive cellular analysis is feasible.

中文翻译：

使用廉价的叠层微流体装置进行细胞即时诊断

细胞分析越来越多地用于在床旁和全球医疗保健机构诊断疾病。一些分析很简单，因为它们依赖于显色染色（血细胞计数、疟疾），但其他分析通常需要更高的多路复用来定义和定量细胞群（癌症诊断、免疫分析）。用廉价的解决方案简化后者代表了当前设计起始管道的瓶颈。基于新型薄膜粘合剂可用于制造廉价的一次性设备的假设，我们测试了许多不同的设计和材料，以快速执行 12-15 通道单细胞成像。使用优化的无源泵送层堆栈微流体 (PLASMIC) 设备（耗材中<1 美元），我们表明快速、廉价的细胞分析是可行的。

更新日期：2022-05-06

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