Artificial incubation is an effective way to increase the supply of red claw crayfish seed. In order to explore the effects of nitrite, ammonia nitrogen and pH on the eggs and nitrite on juvenile during in artificial incubation, four experiments were carried out. The results of Experiment I showed: (1) the hatching rate was less affected by nitrite, while the survival rate was greatly affected by nitrite. There were significant differences among the groups. (2) The analysis of the cubic regression equation showed a downward trend with increasing nitrite concentration. The results of Experiment II showed that the hatching rate was less affected by ammonia nitrogen, while the survival rate was affected by ammonia nitrogen, the suitable value range was 0–0.6 mg/L. The results of Experiment III showed that the incubation rate was less affected by pH, while the survival rate was affected by pH. The survival rate of the pH 7.7 or pH 8.4 group was significantly higher than that of the other three groups. The results of Experiment IV showed: (1) the LC50 of nitrite for 96 h was 11.43 mg/L, and the safe SC concentration was 1.14 mg/L. (2) The survival and growth rate, antioxidant capacity and immune-related indexes of juveniles in the 0.023 mg/L nitrite concentration group were the lowest. In summary, to obtain a stable and high production of red claw crayfish by artificial incubation, it was necessary to keep the concentration of nitrite, ammonia nitrogen and pH value in the appropriate range.

中文翻译：

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氨氮、亚硝酸盐和pH对红爪螯虾人工孵化卵及幼体生长的影响

人工孵化是增加红爪小龙虾种子供应的有效途径。为探讨人工孵化过程中亚硝酸盐、氨氮和pH对卵和亚硝酸盐对幼鱼的影响，共进行了4个实验。实验一的结果表明：（1）孵化率受亚硝酸盐影响较小，而成活率受亚硝酸盐影响较大。各组之间存在显着差异。(2)三次回归方程分析显示随着亚硝酸盐浓度的增加呈下降趋势。实验二的结果表明，孵化率受氨氮的影响较小，而成活率受氨氮的影响，适宜的取值范围为0～0.6 mg/L。实验 III 的结果表明，孵育速率受 pH 值的影响较小，而存活率受pH影响。pH 7.7或pH 8.4组的存活率明显高于其他三组。实验四结果表明：（1）亚硝酸盐96 h的LC50为11.43 mg/L，安全SC浓度为1.14 mg/L。(2)0.023 mg/L亚硝酸盐浓度组幼鱼的存活生长率、抗氧化能力和免疫相关指标最低。综上所述，要通过人工孵化获得稳定、高产的红爪小龙虾，需要将亚硝酸盐、氨氮和pH值保持在合适的范围内。(1)亚硝酸盐96 h的LC50为11.43 mg/L，安全SC浓度为1.14 mg/L。(2)0.023 mg/L亚硝酸盐浓度组幼鱼的存活生长率、抗氧化能力和免疫相关指标最低。综上所述，要通过人工孵化获得稳定、高产的红爪小龙虾，需要将亚硝酸盐、氨氮和pH值保持在合适的范围内。(1)亚硝酸盐96 h的LC50为11.43 mg/L，安全SC浓度为1.14 mg/L。(2)0.023 mg/L亚硝酸盐浓度组幼鱼的存活生长率、抗氧化能力和免疫相关指标最低。综上所述，要通过人工孵化获得稳定、高产的红爪小龙虾，需要将亚硝酸盐、氨氮和pH值保持在合适的范围内。