The effects of plasma functionalized water (PFW) and its combination with ultrasound (UPFW) on the functional and bioactive properties of small yellow croaker protein hydrolysates (SYPHs) produced from three enzymes were investigated. Fluorescence and UV–Vis spectroscopy indicated that SYPHs tended to unfold with increasing intensity and shift in wavelengths to more flexible conformations under PFW and UPFW treatments. Particle size distribution and microstructure analysis revealed that treatments could disrupt aggregation of protein molecules to increase the roughness, specific surface area, and decrease the particle size of peptides during hydrolysis. The partially denatured structure of SYPHs induced by treatments increased the susceptibility of the fish proteins to exogenous enzymes, thereby accelerating the hydrolytic process to yield peptides with improved solubility, decreased emulsifying and foaming properties, and improved enzyme-specific antioxidant properties. The results revealed that the functionality of SYPHs was influenced by the treatment method and the enzyme type employed.

研究了等离子体功能化水 (PFW) 及其与超声 (UPFW) 的组合对由三种酶产生的小黄鱼蛋白水解物 (SYPHs) 的功能和生物活性特性的影响。荧光和紫外-可见光谱表明，在 PFW 和 UPFW 处理下，SYPH 倾向于随着强度的增加和波长的变化而展开，从而形成更灵活的构象。粒度分布和微观结构分析表明，处理可以破坏蛋白质分子的聚集，以增加粗糙度、比表面积，并在水解过程中减小肽的粒度。处理诱导的 SYPHs 的部分变性结构增加了鱼蛋白对外源酶的敏感性，从而加速水解过程以产生具有改善的溶解度、降低的乳化和起泡性质以及改善的酶特异性抗氧化性质的肽。结果表明，SYPHs 的功能受处理方法和所用酶类型的影响。