Proximity labeling can be defined as an enzymatic “in-cell” chemical reaction that catalyzes the proximity-dependent modification of biomolecules in live cells. Since the modified proteins can be isolated and identified via mass spectrometry, this method has been successfully utilized for the characterization of local proteomes such as the sub-mitochondrial proteome and the proteome at membrane contact sites, or spatiotemporal interactome information in live cells, which are not “accessible” via conventional methods. Currently, proximity labeling techniques can be applied not only for local proteome mapping but also for profiling local RNA and DNA, in addition to showing great potential for elucidating spatial cell–cell interaction networks in live animal models. We believe that proximity labeling has emerged as an essential tool in “spatiomics,” that is, for the extraction of spatially distributed biological information in a cell or organism.

中文翻译：

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接近标记的分子空间学

邻近标记可以定义为一种酶促“细胞内”化学反应，它催化活细胞中生物分子的邻近依赖性修饰。由于修饰的蛋白质可以通过质谱分离和鉴定，该方法已成功地用于表征局部蛋白质组，例如亚线粒体蛋白质组和膜接触位点的蛋白质组，或活细胞中的时空相互作用组信息，不能通过传统方法“访问”。目前，邻近标记技术不仅可以应用于局部蛋白质组图谱，还可以用于分析局部 RNA 和 DNA，此外还显示出在活体动物模型中阐明空间细胞-细胞相互作用网络的巨大潜力。我们相信，邻近标签已经成为“空间组学”中必不可少的工具，