The roles of microorganisms in enhancing crop production have been demonstrated for a range of cropping systems. Most studies to date, however, have been confined to a limited number of locations, making it difficult to identify general soil biotic and abiotic characteristics underpinning the yield-promotion across various locations. This knowledge gap limits our capacity to harness soil microbiome to improve crop production. Here we used high-throughput amplicon sequencing to investigate the common features of bacterial community composition, ecological networks and physicochemical properties in six yield-invigorating and adjacent yield-debilitating orchards. We found that yield-invigorating soils exhibited higher contents of organic matter than yield-debilitating soils and harbored unique bacterial communities. Greater alpha diversity and higher relative abundances of Planctomycetota and Chloroflexota were observed in yield-debilitating soils. Co-occurrence network analysis revealed that yield-invigorating soils displayed a greater number of functionally interrelated modules (meta-modules) and a higher proportion of negative links to positive links. Chloroflexota was recognized as a keystone taxon in manipulating the interaction of bacterial communities in yield-invigorating soils. Altogether, we provide evidence that yield-invigorating soils across a range of locations appear to share common features, including accumulation of soil organic matter, higher microbial diversity, enrichment of key taxa like Chloroflexota and maintaining a competitive network. These findings have implications for science-based guidance for sustainable food production.

中文翻译：

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有机质输入引发的土壤细菌群落与梨的高产生产有关

微生物在提高作物产量方面的作用已在一系列种植系统中得到证实。然而，迄今为止的大多数研究都仅限于有限数量的地点，因此难以确定支持不同地点增产的一般土壤生物和非生物特征。这种知识差距限制了我们利用土壤微生物组来改善作物生产的能力。在这里，我们使用高通量扩增子测序研究了六个增产和相邻减产果园的细菌群落组成、生态网络和物理化学性质的共同特征。我们发现，与减产土壤相比，增产土壤的有机质含量更高，并且拥有独特的细菌群落。在减产土壤中观察到更大的 alpha 多样性和更高的 Planctomycetota 和 Chloroflexota 相对丰度。共现网络分析表明，提高产量的土壤显示出更多的功能相关模块（元模块）和更高比例的负链接与正链接。Chloroflexota 被认为是控制增产土壤中细菌群落相互作用的关键分类单元。总而言之，我们提供的证据表明，一系列地区的增产土壤似乎具有共同的特征，包括土壤有机质的积累、更高的微生物多样性、丰富的关键分类群（如 Chloroflexota）和保持竞争网络。这些发现对可持续粮食生产的科学指导具有重要意义。