Lab-on-a-Molecule Probe: Multitarget Detection of Five Aromatic Pesticides Using a Supramolecular Probe under Single Wavelength Excitation,Journal of Agricultural and Food Chemistry

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Lab-on-a-Molecule Probe: Multitarget Detection of Five Aromatic Pesticides Using a Supramolecular Probe under Single Wavelength Excitation
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-05-04 , DOI: 10.1021/acs.jafc.2c00655
Kai-Ni Wei ₁ , Gui-Xian Song ₂ , Shu-Zhen Huang ₁ , Qing Tang ₃ , Jian-Hang Hu ₁ , Zhu Tao ₁ , Ying Huang _{1,

4}

Affiliation

In order to prevent and control the effects of pesticide residues on human health and the ecological environment, the rapid, highly sensitive, and selective detection of multiple pesticide residues has become an urgent problem to be solved. Herein, a lab-on-a-molecule probe based on a host–guest complex (ThT@Q[8] probe) has been developed to simultaneously analyze multiple aromatic pesticides under single wavelength excitation, such as fuberidazole, thiabendazole, carbendazim, thidiazuron, and tricyclazole. The fluorescence titration spectra of the ThT@Q[8] probe with the five pesticides mentioned above showed that the fluorescence intensity exhibited a good linear correlation with the pesticide concentration and the limit of detection was as low as 10^–7 M. Because the ThT@Q[8] probe exhibits diverse fluorescence color changes to the five pesticides studied under a 365 nm ultraviolet lamp, we fabricated a single probe used to detect multiple analytes in the RGB triple channel by extracting the RGB variations. Principal component analysis and linear discriminant analysis proved that the ThT@Q[8] probe can recognize and distinguish five pesticides and can be applied at different concentrations. In real samples, the ThT@Q[8] probe recognized and distinguished five pesticides in tap water and Huaxi River water. The ¹H NMR spectra results proved that a charge-transfer complex of ThT and pesticides in the Q[8] cavity may be formed. Moreover, we selected a test strip as a carrier to detect pesticides. The results indicate it can be used to quickly and conveniently detect different pesticides due to the rapid color change. Besides, the ThT@Q[8] probe has good cell permeability and can be used to detect pesticide residues in living cells. This work has laid the foundation for the qualitative and quantitative multitarget detection of pesticide residues.

中文翻译：

Lab-on-a-Molecule Probe：在单波长激发下使用超分子探针对五种芳香族农药进行多目标检测

为防控农药残留对人体健康和生态环境的影响，对多种农药残留进行快速、高灵敏度、选择性检测已成为亟待解决的问题。在此，开发了一种基于主客体复合物的分子实验室探针（ThT@Q[8] 探针），用于在单波长激发下同时分析多种芳香族农药，例如氟贝立唑、噻苯达唑、多菌灵、噻二唑脲，和三环唑。ThT@Q[8]探针对上述五种农药的荧光滴定光谱表明，荧光强度与农药浓度呈良好的线性相关性，检出限低至^10-7M. 由于 ThT@Q[8] 探针在 365 nm 紫外灯下研究的五种农药显示出不同的荧光颜色变化，因此我们制作了一个探针，用于通过提取 RGB 变化来检测 RGB 三通道中的多种分析物。主成分分析和线性判别分析证明，ThT@Q[8]探针可以识别和区分五种农药，可以在不同浓度下应用。在实际样品中，ThT@Q[8] 探针识别并区分了自来水和花溪河水中的五种农药。¹ _H NMR光谱结果证明，在Q[8]腔中可能形成ThT和农药的电荷转移络合物。此外，我们选择了试纸作为载体来检测农药。结果表明，由于颜色变化快，可用于快速方便地检测不同农药。此外，ThT@Q[8]探针具有良好的细胞渗透性，可用于检测活细胞中的农药残留。该工作为农药残留的定性定量多目标检测奠定了基础。

更新日期：2022-05-04

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