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The impact of dromedary camel milk on mice gut microbiota
Applied Biological Chemistry ( IF 3.2 ) Pub Date : 2022-05-04 , DOI: 10.1186/s13765-022-00699-6
Abdullah Sheikh 1 , Faisal Almathen 1, 2 , Mohammed Alfattah 1
Affiliation  

The gut microbiota plays an important role in the health and disease resistance of the host. Host health depends on the microbiota of the gastrointestinal tract, and imbalance in its composition may leads to certain diseases. This study analyzed the influence that dromedary camel milk has on the gut microbiota of mice. This study characterized the bacterial populations of untreated [Control (C) and camel milk-treated Raw (R), Pasteurized (P), and Fermented (F)] C57BL/6 J mice feces using high-throughput 16S rDNA sequencing on the Illumina MiSeq platform. In total, 286,606 tags were generated, with an average of 71,651 tags being generated per group, and these tags were clustered to the operational taxonomic units (OTUs) at 97% sequence similarity, resulting 1090 OTUs. Significant weight gain was observed among all of the groups, and the total cholesterol level declined in F group followed by in group P compared to group C. The F and P groups demonstrated a correlation between the beneficial microbiota structures that corresponded with lower cholesterol levels than those observed in the other groups. The major dominant bacteria correspond to the phyla Bacteroidetes and Firmicutes. The dromedary camel milk propagated the beneficial bacteria (Allobaculum and Akkermansia) and reduced harmful bacteria such as Proteobacteria, Erysipelotrichaceae, and Desulfovibrionaceae. This study provides a comparative analysis of the gut microbiota of mice based on camel milk, which may be helpful in understanding host health and diverse gut microbial conditions.

中文翻译:

单峰骆驼奶对小鼠肠道菌群的影响

肠道菌群对宿主的健康和抗病能力起着重要作用。宿主的健康取决于胃肠道的微生物群,其组成的不平衡可能导致某些疾病。本研究分析了单峰骆驼奶对小鼠肠道菌群的影响。本研究使用 Illumina 上的高通量 16S rDNA 测序对未经处理的 [对照 (C) 和骆驼奶处理的生 (R)、巴氏杀菌 (P) 和发酵 (F)] C57BL/6 J 小鼠粪便的细菌种群进行了表征MiSeq 平台。总共生成了 286,606 个标签,每组平均生成 71,651 个标签,这些标签以 97% 的序列相似性聚类到操作分类单元 (OTU),得到 1090 个 OTU。在所有组中观察到显着的体重增加,与 C 组相比,F 组的总胆固醇水平下降,其次是 P 组。F 组和 P 组证明了有益微生物群结构之间的相关性,与其他组观察到的胆固醇水平相对应。主要的优势细菌对应于拟杆菌门和厚壁菌门。单峰骆驼奶繁殖了有益细菌(Allobaculum 和 Akkermansia),并减少了变形杆菌、丹毒科和脱硫弧菌科等有害细菌。这项研究提供了基于骆驼奶的小鼠肠道微生物群的比较分析,这可能有助于了解宿主健康和不同的肠道微生物状况。F 组和 P 组证明了有益微生物群结构之间的相关性,这些结构与在其他组中观察到的胆固醇水平较低相对应。主要的优势细菌对应于拟杆菌门和厚壁菌门。单峰骆驼奶繁殖了有益细菌(Allobaculum 和 Akkermansia),并减少了变形杆菌、丹毒科和脱硫弧菌科等有害细菌。这项研究提供了基于骆驼奶的小鼠肠道微生物群的比较分析,这可能有助于了解宿主健康和不同的肠道微生物状况。F 组和 P 组证明了有益微生物群结构之间的相关性,这些结构与在其他组中观察到的胆固醇水平较低相对应。主要的优势细菌对应于拟杆菌门和厚壁菌门。单峰骆驼奶繁殖了有益细菌(Allobaculum 和 Akkermansia),并减少了变形杆菌、丹毒科和脱硫弧菌科等有害细菌。这项研究提供了基于骆驼奶的小鼠肠道微生物群的比较分析,这可能有助于了解宿主健康和不同的肠道微生物状况。单峰骆驼奶繁殖了有益细菌(Allobaculum 和 Akkermansia),减少了变形杆菌、丹毒菌科和脱硫弧菌科等有害细菌。这项研究提供了基于骆驼奶的小鼠肠道微生物群的比较分析,这可能有助于了解宿主健康和多样化的肠道微生物状况。单峰骆驼奶繁殖了有益细菌(Allobaculum 和 Akkermansia),减少了变形杆菌、丹毒菌科和脱硫弧菌科等有害细菌。这项研究提供了基于骆驼奶的小鼠肠道微生物群的比较分析,这可能有助于了解宿主健康和多样化的肠道微生物状况。
更新日期:2022-05-04
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