Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2022-05-04 , DOI: 10.1016/j.saa.2022.121333 Jae Kyong Kim 1 , So Yeon Bong 1 , Rackhyun Park 2 , Junsoo Park 2 , Doo Ok Jang 1
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A probe with an isothiocyanate group was synthesized and evaluated for its H2S sensing ability. Upon addition of H2S, the probe exhibited ratiometric properties during absorption with a red-shift. The probe exhibited fluorescent off–on responses towards H2S via the ESIPT process, due to the conversion of isocyanate into amine. UV–vis, fluorescence, and 1H NMR spectroscopic analyses were performed to investigate the sensing mechanism. The probe has a large Stokes shift, short response time, and low detection limit. It can be used to estimate H2S levels within the range of 0–36 nM. The practical applicability of the probe was demonstrated using water samples and living cells.
中文翻译：
用于检测环境和生物系统中硫化氢的异硫氰酸酯基于 ESIPT 的荧光开启探针
合成具有异硫氰酸酯基团的探针并评估其H 2 S 传感能力。在添加 H 2 S 后，探针在吸收过程中表现出红移的比例特性。由于异氰酸酯转化为胺，探针通过 ESIPT 过程对 H 2 S表现出荧光关闭响应。进行紫外-可见、荧光和1 H NMR 光谱分析以研究传感机制。该探头具有大的斯托克斯位移、短的响应时间和低检测限。它可用于估计0-36 nM 范围内的H 2 S 水平。使用水样和活细胞证明了探针的实际适用性。
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