Electrically-driven handling of gametes and embryos: taking a step towards the future of ARTs,Lab on a Chip

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Electrically-driven handling of gametes and embryos: taking a step towards the future of ARTs
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-05-02 , DOI: 10.1039/d1lc01160j
Adriana Karcz _{1,

2} , Ann Van Soom ₂ , Katrien Smits ₂ , Rik Verplancke ₁ , Sandra Van Vlierberghe ₃ , Jan Vanfleteren ₁

Affiliation

Electrical stimulation of gametes and embryos and on-chip manipulation of microdroplets of culture medium serve as promising tools for assisted reproductive technologies (ARTs). Thus far, dielectrophoresis (DEP), electrorotation (ER) and electrowetting on dielectric (EWOD) proved compatible with most laboratory procedures offered by ARTs. Positioning, entrapment and selection of reproductive cells can be achieved with DEP and ER, while EWOD provides the dynamic microenvironment of a developing embryo to better mimic the functions of the oviduct. Furthermore, these techniques are applicable for the assessment of the developmental competence of a mammalian embryo in vitro. Such research paves the way towards the amelioration and full automation of the assisted reproduction methods. This article aims to provide a summary on the recent developments regarding electrically stimulated lab-on-chip devices and their application for the manipulation of gametes and embryos in vitro.

中文翻译：

配子和胚胎的电动处理：迈向 ART 的未来

配子和胚胎的电刺激以及培养基微滴的片上操作可作为辅助生殖技术 (ART) 的有前途的工具。迄今为止，介电电泳 (DEP)、电旋转 (ER) 和电介质电润湿 (EWOD) 证明与 ART 提供的大多数实验室程序兼容。DEP 和 ER 可以实现生殖细胞的定位、捕获和选择，而 EWOD 提供发育中胚胎的动态微环境，以更好地模拟输卵管的功能。此外，这些技术适用于评估哺乳动物胚胎的体外发育能力. 这样的研究为辅助生殖方法的改进和完全自动化铺平了道路。本文旨在总结电刺激片上实验室设备的最新发展及其在体外操纵配子和胚胎的应用。

更新日期：2022-05-05

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