Maize lethal necrosis (MLN) is a devastating synergistic viral disease of maize caused by maize chlorotic mottle virus (MCMV) plus a virus in the Potyviridae family. The disease has severely impacted maize production in East Africa, Asia, and South America over the past decade, and the presence of MCMV and maize infecting potyviruses in the United States is an ongoing concern. In this study, we performed a genome-wide association study (GWAS) using the Maize 282 Association Mapping Panel (AMP) to characterize the genetic architecture of host response to MCMV and to identify disease-tolerant lines. The GWAS analysis detected genomic variants on chromosomes 3, 4, 5, and 8 that were significantly associated with response to MCMV. Several lines in the 282 AMP were highly tolerant to infection, including CML333, one of the parental lines used in the maize nested association mapping population. Quantitative trait locus analysis of the B73 × CML333 recombinant inbred line population revealed an MCMV tolerance locus on chromosome 10. These findings further our understanding of the genetic architecture of MCMV tolerance and will facilitate the development of maize lines, hybrids, and cultivars with improved resistance to MLN and MCMV.

中文翻译：

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在玉米 282 关联面板中绘制玉米褪绿斑驳病毒耐受基因座

玉米致死性坏死 (MLN) 是由玉米褪绿斑驳病毒 (MCMV) 和马铃薯病毒科中的一种病毒引起的玉米毁灭性协同病毒病。家庭。在过去的十年里，这种疾病严重影响了东非、亚洲和南美洲的玉米生产，美国存在 MCMV 和感染玉米的马铃薯病毒是一个持续关注的问题。在这项研究中，我们使用玉米 282 关联映射面板 (AMP) 进行了全基因组关联研究 (GWAS)，以表征宿主对 MCMV 反应的遗传结构并确定耐病系。GWAS 分析检测到与对 MCMV 的反应显着相关的 3、4、5 和 8 号染色体上的基因组变异。282 AMP 中的几个品系对感染具有高度耐受性，包括 CML333，它是玉米嵌套关联作图群体中使用的亲本品系之一。