Pseudomonas strains are a promising host cell in metabolic engineering for bioconversion, environmental remediation, and most recently for bioelectrochemical applications. This study isolated an electrochemically active Pseudomonas sp. from an anaerobic sludge using a colorimetric and electrochromic WO₃ nanorod (WO₃-NR) probe. A strategy was developed to determine the presence of electroactive species from enriched cultures. A mixed consortium was enriched using Pseudomonas isolation media containing betaine and triclosan as the carbon source and antibacterial reagent, respectively. A single blue colony was isolated using WO₃-NR sandwiched agar plates. The isolate was sequenced by 16 s rRNA and designated Pseudomonas aeruginosa PBH03, producing phenazines and pyocyanin aerobically. The isolate exhibited clear electrochemical characteristics from cyclic voltammetry and linear sweep voltammetry and produced a current density of 9.01 µA cm⁻² in a microbial fuel cell.

假单胞菌菌株是代谢工程中用于生物转化、环境修复以及最近用于生物电化学应用的有前途的宿主细胞。本研究分离出一种具有电化学活性的假单胞菌属。_{使用比色和电致变色 WO 3}纳米棒 (WO ₃ -NR) 探针从厌氧污泥中提取。制定了一种策略来确定富集培养物中是否存在电活性物质。使用分别含有甜菜碱和三氯生作为碳源和抗菌试剂的假单胞菌分离培养基富集混合聚生体。_{使用 WO 3}分离出一个蓝色菌落-NR 夹着琼脂板。通过 16 s rRNA 对分离物进行测序，并将其命名为铜绿假单胞菌PBH03，以需氧方式产生吩嗪和绿脓杆菌素。该分离物在循环伏安法和线性扫描伏安法中表现出明显的电化学特性，并在微生物燃料电池中产生了 9.01 µA cm ^{-2的电流密度。}