The role of the biointerface design towards the development of an impedimetric biosensor for the electrochemical detection of human epidermal growth factor receptor 2 (HER2) is investigated. Two novel cobalt phthalocyanines: cobalt tetraphenyl acetic acid phthalocyanine and cobalt tetraphenyl propionic acid phthalocyanine are compared as signal amplifiers and immobilization platforms of the HB5 aptamer towards the electrochemical detection of HER2. In addition, the phthalocyanines are coupled with the metal based cerium oxide nanoparticles. The efficiency of each electrode modification step and the performance of the constructed aptasensors were assessed by impedance spectroscopy. The aptasensors showed very low limit of detection values (all less than 0.2 ng/mL) with high sensitivity and stability. Furthermore, the aptasensors showed very good performance even in human serum samples. Considering these results, the aptasensors demonstrate great potential for improved monitoring of human epidermal growth factor receptor 2 levels for the management of breast cancers.

研究了生物界面设计在开发用于电化学检测人表皮生长因子受体 2 (HER2) 的阻抗生物传感器中的作用。比较了两种新型钴酞菁：四苯乙酸钴酞菁和四苯丙酸钴酞菁作为 HB5 适体的信号放大器和固定平台，用于 HER2 的电化学检测。此外，酞菁与金属基氧化铈纳米颗粒偶联。通过阻抗谱评估每个电极修饰步骤的效率和构建的适体传感器的性能。适体传感器显示出非常低的检测值（均小于 0.2 ng/mL），具有高灵敏度和稳定性。此外，适体传感器即使在人类血清样本中也表现出非常好的性能。考虑到这些结果，适配体传感器在改进监测人类表皮生长因子受体 2 水平以治疗乳腺癌方面表现出巨大的潜力。