The objective of this study was to evaluate the effects of soilless substrate mixtures on blueberry seedling growth under greenhouse conditions and to determine the optimal growth media for soilless blueberry (Vaccinium australe) cultivation. Trials were carried out with two-year-old blueberry seedlings from April to August 2021. Three mixed substrates (PB: peat and bark (1:1, v/v), PR: peat and rice husk (1:1, v/v), and BR: bark and rice husk (1:1, v/v)) were investigated. The results showed that the plant height, stem diameter and chlorophyll content were much higher in PB and PR than in BR. The PB and PR substrates triggered antioxidant system activity and exerted marked positive effects on blueberry seedling growth. The total flavonoid content was greatly increased in the PB group compared with the other two groups. Most element contents were greatly increased with BR compared with PB and PR. Furthermore, metabolomic and transcriptomic analyses revealed the critical responses of metabolic pathways to different substrate treatments in blueberry leaves collected in June. Overall, the metabolomic data revealed 757 metabolites with decreased levels and 1383 metabolites with increased levels. The levels of multiple prenol lipids, flavonoids, organooxygen compounds, carboxylic acids and derivatives were changed. “Flavonoid biosynthesis” was the most significantly enriched pathway and may be closely related to blueberry growth. Integrated transcriptomic and metabolomic data suggested that the expression of 4 DEGs, VcPAL, VcHCT, VcF14G24.3, and VcCHS, was strongly correlated with flavonoid biosynthesis. This work provides insights for elucidating the mechanisms that mediate the growth adaptability of blueberry cv. ‘Zhaixuan 7’ in different substrates.

本研究的目的是评估无土基质混合物对温室条件下蓝莓幼苗生长的影响，并确定无土蓝莓 ( Vaccinium australe ) 的最佳生长培养基。) 栽培。试验于 2021 年 4 月至 2021 年 8 月用 2 年生蓝莓幼苗进行。三种混合基质（PB：泥炭和树皮（1:1，v/v），PR：泥炭和稻壳（1:1，v/ v) 和 BR：树皮和稻壳 (1:1, v/v)) 进行了研究。结果表明，PB和PR的株高、茎粗和叶绿素含量远高于BR。PB和PR底物触发抗氧化系统活性并对蓝莓幼苗生长产生显着的积极影响。与其他两组相比，PB组的总黄酮含量大大增加。与 PB 和 PR 相比，BR 的大多数元素含量都大大增加。此外，代谢组学和转录组学分析揭示了代谢途径对 6 月份收集的蓝莓叶中不同底物处理的关键反应。总体而言，代谢组学数据显示 757 种代谢物水平降低，1383 种代谢物水平升高。改变了多种异戊烯醇脂、黄酮类化合物、有机氧化合物、羧酸及其衍生物的含量。“类黄酮生物合成”是最显着富集的途径，可能与蓝莓的生长密切相关。综合转录组学和代谢组学数据表明，4 个 DEGs 的表达，“类黄酮生物合成”是最显着富集的途径，可能与蓝莓的生长密切相关。综合转录组学和代谢组学数据表明，4 个 DEGs 的表达，“类黄酮生物合成”是最显着富集的途径，可能与蓝莓的生长密切相关。综合转录组学和代谢组学数据表明，4 个 DEGs 的表达，VcPAL、VcHCT、VcF14G24.3和VcCHS与类黄酮生物合成密切相关。这项工作为阐明介导蓝莓品种生长适应性的机制提供了见解。'翟轩7'不同底物。