The aim of this research was to prepare aqueous and ethanolic extracts of the seed and leaf of the wild chestnut known as Ohrid diamond and to determine the antioxidant and antimicrobial activity. In general, ethanolic extracts showed higher antioxidant potential compared with the aqueous extracts. Moreover, ethanolic seed extracts had better results in all of the antioxidant tests compared with the ethanolic extracts from the leaves. The ethanolic seed extract was characterized by a statistically significant (p < 0.05) lower IC₅₀ value for the ability to capture DPPH radicals (0.63 mg/ml) compared with all other tested extracts. Furthermore, the extracts showed statistically significantly (p < 0.05) higher antimicrobial activity against five tested bacteria (Staphylococcus aureus^ATCC25923, Listeria monocytogenes^ATCC19115, Escherichia coli^ATCC11230, Shigella sonnei^ATCC29930, Pseudomonas aeruginosa^ATCC35554) compared to tetracycline and chloramphenicol. Given the good biological activity that was established, this research can serve as a novel starting point to contribute to greater utilization of this plant in the pharmaceutical, cosmetic, and food industries, and the obtained extracts could substitute for the numerous synthetic additives that are regularly used in these industries.

这项研究的目的是制备被称为奥赫里德钻石的野生栗子的种子和叶子的水提取物和乙醇提取物，并确定其抗氧化和抗菌活性。一般来说，与水提取物相比，乙醇提取物显示出更高的抗氧化潜力。此外，与叶子的乙醇提取物相比，乙醇种子提取物在所有抗氧化测试中的结果都更好。 与所有其他测试的提取物相比，乙醇种子提取物的特征在于捕获 DPPH 自由基的能力 (0.63 mg/ml) 的IC ₅₀值较低，具有统计学意义 ( p < 0.05)。此外，提取物在统计学上显示出显着性（p< 0.05)与四环素和氯霉素相比， 对五种测试细菌（金黄色葡萄球菌^ATCC25923、单核细胞增生李斯特菌^ATCC19115、大肠杆菌^ATCC11230、宋内志贺氏菌^ATCC29930、铜绿假单胞菌^{ATCC35554 ）的抗菌活性更高。}鉴于已建立的良好生物活性，这项研究可以作为一个新的起点，以促进该植物在制药、化妆品和食品工业中的更大利用，并且获得的提取物可以替代许多常规的合成添加剂用于这些行业。