White lupin (Lupinus albus L.) is a promising crop to meet the rising global demand for plant-based protein. The seed-borne pathogen Colletotrichum lupini, however, threatens lupin cultivation worldwide. Seed dressings using synthetic fungicides were shown effective to reduce infection levels, but their negative environmental impact and exclusion from organic production calls for more sustainable solutions. In this study, a total of eleven different alternative seed treatments were tested in field trials in Switzerland between 2018 and 2021. Treatment types consisted of: hot water, steam, electron, long-term storage, elevated partial pressure of oxygen (EPPO), vinegar, plant extracts and biological control agents (BCAs). The BCAs were tested for potential antagonistic activity against C. lupini during white lupin infection under controlled conditions prior to field trials. Long-term storage and vinegar treatments successfully reduced disease incidence and increased yield to levels similar to those observed for certified seeds, without significantly affecting germination rate. Although promising, effectiveness of these treatments needs further validation. Four BCAs showed significant disease reductions under controlled conditions. Besides lowering disease severity, two BCAs also reduced C. lupini DNA in stem tissue. These reductions, however, were not observed in the field, highlighting the importance of field validations. The treatments identified in this study provide a solid basis for the development of sustainable and effective seed protection strategies in white lupin to control C. lupini successfully.

白羽扇豆 ( Lupinus albus L.) 是一种很有前途的作物，可以满足全球对植物蛋白不断增长的需求。种子传播病原体Colletotrichum lupini然而，威胁着全世界的羽扇豆种植。使用合成杀菌剂的拌种剂被证明可以有效降低感染水平，但它们对环境的负面影响和被排除在有机生产之外需要更可持续的解决方案。在这项研究中，2018 年至 2021 年间在瑞士的田间试验中总共测试了 11 种不同的替代种子处理。处理类型包括：热水、蒸汽、电子、长期储存、提高氧分压 (EPPO)、醋、植物提取物和生物控制剂 (BCA)。测试了 BCA 对C. lupini的潜在拮抗活性在田间试验前在受控条件下的白羽扇豆感染期间。长期储存和醋处理成功地降低了疾病的发生率，并将产量提高到与经过认证的种子相似的水平，而不会显着影响发芽率。虽然很有希望，但这些治疗的有效性需要进一步验证。四个 BCA 在受控条件下显示出显着的疾病减少。除了降低疾病严重程度外，两种 BCA 还减少了茎组织中的C. lupini DNA。然而，在现场没有观察到这些减少，突出了现场验证的重要性。本研究中确定的处理方法为制定可持续和有效的白羽扇豆种子保护策略以控制C. lupini成功。