In order to elucidate the contributions of JA in orchestrating disease resistance in potato plants, the potato genotype ‘SD20’, which exhibits strong resistance against the highly virulent Phytophthora infestans isolate CN152, while infected by the super virulent isolate 2013-18-306, was treated with exogenous JA and then challenged by inoculation with 2013-18-306. The results showed that exogenously applied JA significantly delayed the onset and alleviated the symptoms of late blight, indicating exogenous JA could induce resistance to P. infestans in the early biotrophic stage of infection in ‘SD20’ plants. To further clarify the role of JA in the early defense response and identify key genes involved in JA signal transduction, gene expression profiling via RNA sequencing (RNA-seq) in ‘SD20’ plants treated with exogenously applied JA was performed. A total of 2 927 differentially expressed genes were specifically induced, the majority encoded transcription factors, protein kinases, secondary metabolites, defense enzymes and disease resistance related proteins. GO functional annotation and KEGG metabolic pathway analysis showed that exogenously applied JA rapidly induced the expression of genes related to immune response regulation, pathogen defense, and other biological processes, and stimulated endogenous JA synthesis and signal transduction, and the overall early pathogen defense response in ‘SD20’. These results provide useful information in understanding the JA's function involved in pathogen defense responses and a theoretical basis for the application of JA in potato production.

为了阐明 JA 在协调马铃薯植物抗病性中的贡献，马铃薯基因型“SD20”在被超强毒力分离物 2013-18-306 感染的同时对高毒力致病疫霉分离物 CN152 表现出强抗性，是用外源 JA 处理，然后用 2013-18-306 接种攻击。结果表明，外源JA显着延缓了晚疫病的发病，减轻了晚疫病症状，表明外源JA可诱导对致病疫霉的抗性。在“SD20”植物感染的早期生物营养阶段。为了进一步阐明 JA 在早期防御反应中的作用并确定参与 JA 信号转导的关键基因，通过 RNA 测序 (RNA-seq) 在用外源应用 JA 处理的“SD20”植物中进行基因表达谱分析。共特异性诱导了2 927个差异表达基因，其中大部分编码转录因子、蛋白激酶、次级代谢产物、防御酶和抗病相关蛋白。GO功能注释和KEGG代谢通路分析表明，外源JA快速诱导免疫反应调节、病原体防御等生物学过程相关基因的表达，并刺激内源JA合成和信号转导，以及“SD20”中的整体早期病原体防御反应。这些结果为了解JA参与病原体防御反应的功能提供了有用的信息，并为JA在马铃薯生产中的应用提供了理论基础。