A novel, integrated process for the production of ethanol from lignocellulosic biomass is reported which involves simultaneous pretreatment, saccharification and fermentation using environmentally friendly technologies. The lignocellulosic biomass (8.33 g/L rice straw) was pretreated and saccharified with the anaerobic fungus Pecoramyces sp. F1, while the facultative anaerobic bacterium Zymomonas mobilis ATCC 31821 was employed to simultaneously ferment to produce ethanol. Without the need for additional pretreatment, the conversion from lignocellulosic biomass to ethanol was completed in four days with a production yield of 0.32 g ethanol/g glucose and a volumetric productivity of 0.010 g/L/h (0.98 g/L). Meanwhile, other metabolites were produced, including 0.024 g/L/h (2.28 g/L) acetate, 0.002 g/L/h (0.22 g/L) lactate and 0.002 g/L/h (0.15 g/L) formate. Results showed that the integrated process has the potential for ethanol production from lignocellulosic biomass.

报道了一种从木质纤维素生物质生产乙醇的新型集成工艺，该工艺涉及使用环保技术同时进行预处理、糖化和发酵。木质纤维素生物质（8.33 g/L 稻草）用厌氧真菌Pecoramyces sp. 预处理和糖化。F1，兼性厌氧菌运动发酵单胞菌ATCC 31821 用于同时发酵生产乙醇。无需额外的预处理，从木质纤维素生物质到乙醇的转化在四天内完成，产量为 0.32 g 乙醇/g 葡萄糖，体积生产率为 0.010 g/L/h (0.98 g/L)。同时，产生了其他代谢物，包括 0.024 g/L/h (2.28 g/L) 乙酸盐、0.002 g/L/h (0.22 g/L) 乳酸和 0.002 g/L/h (0.15 g/L) 甲酸盐。结果表明，该集成工艺具有利用木质纤维素生物质生产乙醇的潜力。