Overexpression of cytoplasmic C4 Flaveria bidentis carbonic anhydrase in C3 Arabidopsis thaliana increases amino acids, photosynthetic potential, and biomass,Plant Biotechnology Journal

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Overexpression of cytoplasmic C4 Flaveria bidentis carbonic anhydrase in C3 Arabidopsis thaliana increases amino acids, photosynthetic potential, and biomass
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2022-04-25 , DOI: 10.1111/pbi.13830
Deepika Kandoi ₁ , Kamal Ruhil ₁ , Govindjee Govindjee ₂ , Baishnab C Tripathy _{1,

3}

Affiliation

An important method to improve photosynthesis in C₃ crops, such as rice and wheat, is to transfer efficient C₄ characters to them. Here, cytosolic carbonic anhydrase (CA: βCA3) of the C₄ Flaveria bidentis (Fb) was overexpressed under the control of ³⁵S promoter in Arabidopsis thaliana, a C₃ plant, to enhance its photosynthetic efficiency. Overexpression of CA resulted in a better supply of the substrate

{HCO}_{3}^{-}

for the endogenous phosphoenolpyruvate carboxylase in the cytosol of the overexpressers, and increased its activity for generating malate that feeds into the tricarboxylic acid cycle. This provided additional carbon skeleton for increased synthesis of amino acids aspartate, asparagine, glutamate, and glutamine. Increased amino acids contributed to higher protein content in the transgenics. Furthermore, expression of FbβCA3 in Arabidopsis led to a better growth due to expression of several genes leading to higher chlorophyll content, electron transport, and photosynthetic carbon assimilation in the transformants. Enhanced CO₂ assimilation resulted in increased sugar and starch content, and plant dry weight. In addition, transgenic plants had lower stomatal conductance, reduced transpiration rate, and higher water-use efficiency. These results, taken together, show that expression of C₄ CA in the cytosol of a C₃ plant can indeed improve its photosynthetic capacity with enhanced water-use efficiency.

中文翻译：

C3 拟南芥中细胞质 C4 Flaveria bidentis 碳酸酐酶的过表达增加了氨基酸、光合潜力和生物量

提高水稻、小麦等C _{3作物光合作用的一个重要方法是将高效的C}₄性状传递给它们。在这里， C ₄Flaveria bidentis ( Fb ) 的胞质碳酸酐酶 (CA: βCA3 ) 在C _3植物拟南芥中的³⁵ S 启动子的控制下过表达，以提高其光合效率。CA的过表达导致更好的底物供应

{碳酸氢钠}_{3}^{-}

用于过度表达细胞胞质溶胶中的内源性磷酸烯醇丙酮酸羧化酶，并增加其产生苹果酸的活性，该苹果酸进入三羧酸循环。这为增加氨基酸天冬氨酸、天冬酰胺、谷氨酸和谷氨酰胺的合成提供了额外的碳骨架。增加的氨基酸有助于转基因中更高的蛋白质含量。此外，由于几个基因的表达导致转化体中更高的叶绿素含量、电子传递和光合碳同化，拟南芥中FbβCA3的表达导致了更好的生长。增强的 CO ₂同化导致糖和淀粉含量以及植物干重增加。此外，转基因植物的气孔导度较低，蒸腾速率降低，水分利用效率较高。这些结果综合起来表明，C ₄ CA在 C ₃植物细胞质中的表达确实可以提高其光合能力，同时提高水分利用效率。

更新日期：2022-04-25

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