In this study, it was aimed to demonstrate the short-term effect of breast cancer surgery and tumor removal on the metabolomic profiles of patients with early-stage breast cancer. This cohort consisted of 18 early-stage breast carcinoma patients who had breast cancer surgery to remove tumor and surrounding tissues. The blood samples obtained preoperatively and 24 h after surgery were used in this investigation. Gas chromatography–mass spectrometry (GC–MS) based metabolomic analysis was performed to determine the metabolites. The GC–MS-based metabolomics profile enabled the identification of 162 metabolites in the plasma samples. Postoperatively, glyceric acid, phosphoric acid, O-phosphocolamine, 2-hydroxyethyliminodiacetic acid, N-acetyl-D-mannosamine, N-acetyl-5-hydroxytryptamine, methyl stearate, methyl oleate, iminodiacetic acid, glycerol 1-phosphate, β-glycerol phosphate and aspartic acid were found to be significantly increased (P < 0.05 for all), whereas saccharic acid, leucrose, gluconic acid, citramalic acid and acetol were significantly decreased (P < 0.05 for all). Breast cancer surgery and tumor removal has an impact on the metabolomic profiles of patients with early-stage breast cancer. These findings can be used for understanding the pathogenesis of breast cancer biology and screening the success of the surgery.

中文翻译：

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基于 GC-MS 的代谢组学分析评估肿瘤切除对早期乳腺癌患者的短期效果

在这项研究中，旨在证明乳腺癌手术和肿瘤切除对早期乳腺癌患者代谢组谱的短期影响。该队列由 18 名早期乳腺癌患者组成，他们接受了乳腺癌手术以切除肿瘤和周围组织。本研究使用术前和术后 24 小时采集的血样。进行基于气相色谱-质谱（GC-MS）的代谢组学分析以确定代谢物。基于 GC-MS 的代谢组学图谱能够鉴定血浆样本中的 162 种代谢物。术后使用甘油、磷酸、O-磷酸可胺、2-羟乙基亚氨基二乙酸、N-乙酰基-D-甘露糖胺、N-乙酰基-5-羟色胺、硬脂酸甲酯、油酸甲酯、亚氨基二乙酸、1-磷酸甘油、β-甘油磷酸盐和天冬氨酸显着增加（均P＜0.05），而糖二酸、明串珠菌糖、葡萄糖酸、柠苹果酸和丙酮醇显着降低（均P＜0.05）。乳腺癌手术和肿瘤切除对早期乳腺癌患者的代谢组学特征有影响。这些发现可用于了解乳腺癌生物学的发病机制和筛选手术的成功。