Trichomoniasis, as a major public health concern, is the world’s most common sexually transmissible disease. This infection is caused by a protozoan parasite called Trichomonas vaginalis (TV) that can lead to reproductive and genital area problems. Accordingly, it is of great significance to expand new methods for increasing the sensitivity of TV detection. In this study, a novel label-free electrochemical DNA biosensor for TV quantitation was constructed based on an electropolymerized poly(ortho-aminophenol) thin film simultaneously acting as a transducer as well as a redox indicator. The redox-active polymeric film was utilized to covalent immobilization of a specific thiolated DNA probe. The hybridization process was then monitored by differential pulse voltammetry. The proposed biosensor detected a synthetic TV target sequence with a calibration sensitivity of − 0.0113 µA (log (concentration/mol L⁻¹))⁻¹, a linear concentration range of 1.0 × 10⁻²⁰ to 1.0 × 10⁻¹² mol L⁻¹, and a detection limit of 3.9 × 10⁻²¹ mol L⁻¹. It also showed an ability to differentiate between the complementary sequence and the base-mismatched and non-complementary sequences with a nice selectivity. Moreover, the designed biosensor was able to detect the TV genome with a calibration sensitivity of − 0.0774 µA (log (concentration/ng µL⁻¹))⁻¹, a linear range of 0.55–64 ng mL⁻¹ and a detection limit of 1.0 pg µL⁻¹.

滴虫病作为主要的公共卫生问题，是世界上最常见的性传播疾病。这种感染是由一种叫做阴道毛滴虫的原生动物寄生虫引起的（电视）可能导致生殖和生殖器区域问题。因此，拓展新的方法来提高电视检测的灵敏度具有重要意义。在这项研究中，基于同时作为传感器和氧化还原指示剂的电聚合聚（邻氨基苯酚）薄膜构建了一种用于 TV 定量的新型无标记电化学 DNA 生物传感器。氧化还原活性聚合物膜用于共价固定特定的硫醇化 DNA 探针。然后通过差分脉冲伏安法监测杂交过程。所提出的生物传感器检测到合成 TV 目标序列，其校准灵敏度为 - 0.0113 µA (log (浓度/mol L ^-1 )) ^-1，线性浓度范围为 1.0 × 10 ^-20至1.0 × 10 ^-12 mol L ^-1，检测限为3.9 × 10 ^-21 mol L ^-1。它还表现出以良好的选择性区分互补序列和碱基错配和非互补序列的能力。此外，所设计的生物传感器能够检测 TV 基因组，其校准灵敏度为 - 0.0774 µA (log (浓度/ng µL ^-1 )) ^-1，线性范围为 0.55–64 ng mL ^-1，检测限为1.0 pg µL ^-1。