Background

Alzheimer’s disease (AD) is an age-related neurodegenerative disease and exercises might mitigate the progression of AD. This investigation aimed to manifest the potential mechanism of exercises in AD.

Methods

Morris water maze (MWM) test was conducted to evaluate the cognitive function in APP/PS1 mice. Quantitative real-time PCR was performed to detect the expression of HOTAIR and miR-130a-3p. The enzyme-linked immunosorbent assay was applied to appraise the concentration of IL-1β, IL-6, and TNF-α. A luciferase report experiment was implemented to substantiate the relationship between miR-130a-3p and HOTAIR.

Results

Exercises contributed to the elevated expression of HOTAIR. The findings of MWM implied HOTAIR inhibited the impacts of voluntary exercises on escape latency, distance moved, percentage of time spent in the target quadrant, platform crossing times, and inflammation. MiR-130a-3p mediated the function of HOTAIR on cognitive ability and inflammation.

Conclusion

HOTAIR participated in the regulation of exercises on AD by sponging miR-130a-3p.

中文翻译：

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lncRNA HOTAIR/miR-130a-3p 轴参与自主运动对阿尔茨海默病认知和炎症的调节

 背景

阿尔茨海默病 (AD) 是一种与年龄相关的神经退行性疾病，锻炼可能会减缓 AD 的进展。本研究旨在揭示运动在 AD 中的潜在机制。

 方法

进行莫里斯水迷宫（MWM）测试来评估APP/PS1小鼠的认知功能。实时定量PCR检测HOTAIR和miR-130a-3p的表达。采用酶联免疫吸附法测定IL-1β、IL-6、TNF-α的浓度。进行荧光素酶报告实验来证实 miR-130a-3p 和 HOTAIR 之间的关系。

 结果

锻炼有助于 HOTAIR 表达的升高。 MWM 的研究结果表明，HOTAIR 抑制了自愿锻炼对逃避潜伏期、移动距离、在目标象限中花费的时间百分比、平台穿越时间和炎症的影响。 MiR-130a-3p 介导 HOTAIR 对认知能力和炎症的功能。

 结论

HOTAIR 通过海绵 miR-130a-3p 参与对 AD 的运动调节。