Common scab of potatoes is a soil-borne disease causing considerable economic losses worldwide. Although many management practices to control this disease have been introduced, the lack of efficiency increases an interest in effective and environmentally friendly alternatives. One of the attractive approaches to manage the disease is an application of antagonistic strains into soil. The aim of this study was to develop a selection approach for suitable biocontrol agents based on their in vitro properties and demonstrate their effect on soil microbial communities and host plant nutrition. Out of 56 isolates of Actinobacteria, three isolates and a mixture of six isolates with strong inhibitory activities against common scab pathogens and high growth rates were selected for the pot experiment to assess their biocontrol activity. The microbial community composition of the tuberosphere and the nutrient content in potato leaves was determined. One isolate significantly reduced the severity of the common scab. This isolate showed strong inhibitory activity against pathogens and reached the highest number of CFU/g of vermiculite. In the pot experiment, all the isolates affected the microbial community composition, yet the most substantial changes occurred in the treatment of the most efficient isolate. Moreover, it was determined that inoculation with isolates led to alterations in host plant nutrition suggesting plant-microbe interactions. We provided insights into relationships between antagonistic isolates, common scab pathogens, host plant and microbial communities, and their role in the disease control.

马铃薯结痂病是一种土传病害，在世界范围内造成相当大的经济损失。尽管已经引入了许多控制这种疾病的管理实践，但缺乏效率增加了对有效和环保替代品的兴趣。控制这种疾病的有吸引力的方法之一是将拮抗菌株应用到土壤中。本研究的目的是开发一种基于其体外特性的合适生物防治剂的选择方法，并证明它们对土壤微生物群落和寄主植物营养的影响。在 56 个放线菌分离株中，选择三个分离株和六个分离株的混合物，对常见的赤霉病病原体具有强抑制活性和高生长率，用于盆栽试验，以评估它们的生防活性。测定了块茎圈的微生物群落组成和马铃薯叶片中的养分含量。一种分离物显着降低了普通结痂的严重程度。该分离物对病原体表现出很强的抑制活性，达到最高的CFU/g蛭石数。在盆栽实验中，所有分离株都影响了微生物群落组成，但最显着的变化发生在最有效的分离株的处理上。此外，确定接种分离物会导致宿主植物营养的改变，这表明植物-微生物相互作用。