Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(β-amino ester)s,ACS Macro Letters

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Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(β-amino ester)s
ACS Macro Letters ( IF 5.1 ) Pub Date : 2022-04-20 , DOI: 10.1021/acsmacrolett.2c00095
Qiuxia Li ₁ , Litao Sun ₁ , Xiaobei Huang ₂ , Shuai Liu ₃ , Haiyang Yong ₁ , Chenfei Wang ₁ , Jianzhong Li ₄ , Dezhong Zhou ₁

Affiliation

Biodegradable and lipid-like highly branched poly(β-amino ester)s, HPAESA, were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. Biodegradability reduces the cytotoxicity of HPAESA and enables controlled DNA release. Lipid mimicry enhances cellular uptake and endosomal escape of HPAESA/DNA polyplexes. HPAESA are able to transfect rat adipose-derived stem cells (rADSs) and human ADSCs (hADSCs) with orders of magnitude higher efficiency than commercial gene transfection reagents, with cell viability exceeding 90%. Most importantly, HPAESA can effectively transfer the nerve growth factor (NGF)-encoding plasmid to rADSCs and induce high NGF secretion, which significantly promotes neurite outgrowth of PC12 cells.

中文翻译：

使用可生物降解和类脂质的高度支化聚（β-氨基酯）对脂肪源性干细胞进行基因工程

开发了可生物降解和类脂的高度支化聚（β-氨基酯）HPAESA，以通过基因转染增强脂肪来源干细胞的生物学功能。可生物降解性降低了 HPAESA 的细胞毒性并能够控制 DNA 释放。脂质模拟增强 HPAESA/DNA 复合物的细胞摄取和内体逃逸。HPAESA 能够以比商业基因转染试剂高几个数量级的效率转染大鼠脂肪干细胞 (rADSs) 和人类 ADSCs (hADSCs)，细胞活力超过 90%。最重要的是，HPAESA 可以有效地将编码神经生长因子 (NGF) 的质粒转移到 rADSCs 并诱导高 NGF 分泌，从而显着促进 PC12 细胞的神经突生长。

更新日期：2022-04-20

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