The plant epitranscriptome: revisiting pseudouridine and 2′-O-methyl RNA modifications,Plant Biotechnology Journal

当前位置： X-MOL 学术 › Plant Biotech. J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The plant epitranscriptome: revisiting pseudouridine and 2′-O-methyl RNA modifications
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2022-04-21 , DOI: 10.1111/pbi.13829
Muthusamy Ramakrishnan _{1,

2} , K Shanmugha Rajan _{3,

4} , Sileesh Mullasseri ₅ , Sarin Palakkal ₆ , Krishnan Kalpana ₇ , Anket Sharma ₈ , Mingbing Zhou _{8,

9} , Kunnummal Kurungara Vinod ₁₀ , Subbiah Ramasamy ₁₁ , Qiang Wei _{1,

2}

Affiliation

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, China.
Bamboo Research Institute, Nanjing Forestry University, Nanjing, Jiangsu, China.
The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University, 52900, Ramat-Gan, Israel.
Department of Chemical and Structural Biology, Weizmann Institute, 7610001, Rehovot, Israel.
School of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies, Cochin, India.
The Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
Department of Plant Pathology, Agricultural College and Research Institute, Tamilnadu Agricultural University, 625 104, Madurai, Tamil Nadu, India.
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, Zhejiang, China.
Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-Efficiency Utilization, Zhejiang A&F University, Hangzhou, Zhejiang, China.
Division of Genetics, ICAR - Indian Agricultural Research Institute, New Delhi, India.
Cardiac Metabolic Disease Laboratory, Department of Biochemistry, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India.

There is growing evidence that post-transcriptional RNA modifications are highly dynamic and can be used to improve crop production. Although more than 172 unique types of RNA modifications have been identified throughout the kingdom of life, we are yet to leverage upon the understanding to optimize RNA modifications in crops to improve productivity. The contributions of internal mRNA modifications such as N6-methyladenosine (m⁶A) and 5-methylcytosine (m⁵C) methylations to embryonic development, root development, leaf morphogenesis, flowering, fruit ripening and stress response are sufficiently known, but the roles of the two most abundant RNA modifications, pseudouridine (Ψ) and 2′-O-methylation (Nm), in the cell remain unclear due to insufficient advances in high-throughput technologies in plant development. Therefore, in this review, we discuss the latest methods and insights gained in mapping internal Ψ and Nm and their unique properties in plants and other organisms. In addition, we discuss the limitations that remain in high-throughput technologies for qualitative and quantitative mapping of these RNA modifications and highlight future challenges in regulating the plant epitranscriptome.

中文翻译：

植物表观转录组：重访假尿苷和 2'-O-甲基 RNA 修饰

越来越多的证据表明转录后 RNA 修饰具有高度动态性，可用于提高作物产量。尽管在整个生命王国中已经确定了超过 172 种独特类型的 RNA 修饰，但我们尚未利用这些知识来优化作物中的 RNA 修饰以提高生产力。N6-甲基腺苷 (m ⁶ A) 和 5-甲基胞嘧啶 (m ⁵ C) 甲基化等内部 mRNA 修饰对胚胎发育、根系发育、叶片形态发生、开花、果实成熟和应激反应的贡献已广为人知，但作用两种最丰富的 RNA 修饰，假尿苷 (Ψ) 和 2'- O由于植物发育中高通量技术的进展不足，细胞中的-甲基化（Nm）仍不清楚。因此，在这篇综述中，我们讨论了在绘制内部 Ψ 和 Nm 及其在植物和其他生物体中的独特特性方面的最新方法和见解。此外，我们讨论了高通量技术在对这些 RNA 修饰进行定性和定量映射方面仍然存在的局限性，并强调了未来在调节植物表观转录组方面的挑战。

更新日期：2022-04-21

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11