Anthracnose (Elsinöe ampelina) is a major fungal disease in humid regions, causing damage to grapevine berries and decreasing fruit quality. Studies concerning enzymatic and biochemical changes caused by infection and tissue colonization of E. ampelina in berries are scarce, but they are fundamental to gaining insights about the defense response to the pathogen. Thus, this study aims to quantify biochemical and enzymatic alterations in different varieties of grapevine berries attacked by E. ampelina, and their association with anthracnose symptoms. Must quality, carbohydrate production, and enzymatic activity were assessed in berries with and without anthracnose symptoms, from five varieties that presented different degrees of anthracnose symptoms. Berries with anthracnose symptoms showed higher must acidity, while berries without anthracnose symptoms produced more proteins and higher polyphenol oxidase activity. The varieties manifesting the highest anthracnose symptoms showed higher sugar and hydrogen peroxide concentrations on berry skins. On the other hand, the varieties with the lowest anthracnose symptoms showed the highest catalase, polyphenol oxidase, and phenylalanine ammonia-lyase activities on berry skins. In conclusion, the varieties presented different severities of anthracnose on berries with higher anthracnose severities correlated with high hydrogen peroxide production and carbohydrate concentration on grape skins.

炭疽病（Elsinöe ampelina）是潮湿地区的主要真菌病害，对葡萄浆果造成损害并降低果实品质。关于浆果中E. ampelina感染和组织定植引起的酶学和生化变化的研究很少，但它们对于深入了解对病原体的防御反应至关重要。因此，本研究旨在量化被E. ampelina攻击的不同葡萄浆果品种的生化和酶促变化，以及它们与炭疽病症状的关联。在有和没有炭疽病症状的浆果中，对表现出不同程度炭疽病症状的五个品种进行了葡萄汁质量、碳水化合物产量和酶活性的评估。有炭疽病症状的浆果表现出更高的葡萄汁酸度，而没有炭疽病症状的浆果产生更多的蛋白质和更高的多酚氧化酶活性。表现出最高炭疽病症状的品种在浆果皮上的糖和过氧化氢浓度较高。另一方面，炭疽病症状最低的品种在浆果皮上的过氧化氢酶、多酚氧化酶和苯丙氨酸解氨酶活性最高。综上所述，