Herein, a facile and sensitive dual mode sensing strategy for amantadine (AMD) level evaluation by coupling the plasmonic Au nanorod (NR) and supramolecular SH-cyclodextrin (CD) through strong Au-S bond is proposed. Methylene blue (MB) molecules can be inserted into the cavity of CD molecules through the hydrophobic interaction, which would cause the plasmon resonance energy transfer (PRET) process as well as electrochemical signal response due to the spectrum overlap between Au NR and MB molecules and the electrochemical conversion activity of MB molecules. Subsequently, AMD would induce the replacement of MB molecules because of the stronger interaction with CD, resulting the recovery of scattering intensity of Au NR and decrease of the electrooxidation current of MB. On one hand, the increase of Au NR scattering intensity is linearly proportional to AMD with the concentration from 0.4 to 3.0 μM, and reaches a limit of detection (LOD) of 0.28 μM. On the other hand, electrochemical measurement method enlarged the detection range of AMD. The variation of electrochemical oxidation peak current of MB is linearly proportional to the logarithm value of AMD concentration from 2.5 to 375.0 μM, with LOD of 1.9 μM. Subsequently, the proposed dual mode response sensing strategy was successfully employed for the detection of AMD in human serum samples with great selectivity and sensitivity, with a recovery percentage ranged from 92.6 to 112.0%. Overall, this Au NR@SH-CD-MB nanoparticle based single particle plasmonic and electrochemical dual mode sensing method provides great potential in the field of clinical drug detection or metabolic process investigation in the future.

在此，提出了一种通过强 Au-S 键耦合等离子体 Au 纳米棒 (NR) 和超分子 SH-环糊精 (CD) 来评估金刚烷胺 (AMD) 水平的简便且灵敏的双模式传感策略。亚甲蓝 (MB) 分子可以通过疏水相互作用插入 CD 分子的空腔，由于 Au NR 和 MB 分子之间的光谱重叠，这将导致等离子体共振能量转移 (PRET) 过程以及电化学信号响应和MB分子的电化学转化活性。随后，由于与CD的相互作用更强，AMD会诱导MB分子的置换，导致Au NR的散射强度恢复，MB的电氧化电流降低。一方面，Au NR 散射强度的增加与 AMD 呈线性关系，浓度从 0.4 到 3.0 μM，并达到 0.28 μM 的检测限 (LOD)。另一方面，电化学测量方法扩大了AMD的检测范围。MB的电化学氧化峰电流的变化与AMD浓度的对数值在2.5到375.0 μM之间呈线性关系，LOD为1.9 μM。随后，所提出的双模式响应传感策略成功地用于检测人血清样品中的 AMD，具有很高的选择性和灵敏度，回收率在 92.6% 到 112.0% 之间。总体，