A feeding trial was conducted to assess the effects of nano-selenium (n.Se) supplementation in a plant protein (PP)-based diet on female Acanthopagrus arabicus broodfish and their offspring. Two basal diets were prepared: a fish meal (FM)-based diet (FM-control) as a positive control and a PP-based diet. The PP-based diet was supplemented with n.Se at five levels including 0 (PP-control, as a negative control), 0.5, 1, 2, and 4 mg/ Kg. Brooders were fed for 90 days with one of the six experimental diets. Supplementing the PP-based diet with 2–4 mg n.Se/Kg markedly enhanced survival rate in 3-day post-hatch larvae (3DPH) larvae and total body length in newly born and 3DPH larvae. Serum triiodothyronine (T₃) significantly decreased in females by supplementing PP-based diet with 1–4 mg n.Se/kg, meanwhile, serum thyroxine (T₄) increased in females fed 1–4 mg n.Se/kg PP-based diets. The level of serum T₃ in females fed FM-based diet was lower than those fed PP-based diet without n.Se supplementation (0.6 vs. 1.9 nmol/L⁻¹). Meanwhile, the serum T₄ was markedly higher in broodfish fed the FM-based diet than those fed the PP-based diet (21.9 vs. 14.3 nmol/L⁻¹). The levels of T₃ and T₄ in 3DPH larvae originating from females fed the PP-based diet without n.Se supplementation were lower than in FM-based and n.Se supplemented PP-based groups (P < 0.05). Serum alkaline phosphatase (ALP) increased in females with increasing n.Se level in the PP-based diet. The highest and lowest ALP levels were in 3DPH larvae originating from females fed PP-based diet without n.Se supplementation and those fed with 1 mg n.Se/Kg PP-based diet, respectively (P < 0.05). The results of the present study suggest supplementing 2–4 mg n.Se in a PP-based diet can improve performance in A. arabicus brooders and larvae that are associated with modulation of thyroid hormones in this species.

进行了一项喂养试验，以评估在基于植物蛋白 (PP) 的饮食中补充纳米硒 (n.Se) 对雌性小叶棘鱼及其后代的影响。准备了两种基础日粮：作为阳性对照的基于鱼粉 (FM) 的日粮（FM-对照）和基于 PP 的日粮。以 PP 为基础的饮食中添加了五个水平的 n.Se，包括 0（PP 对照，作为阴性对照）、0.5、1、2 和 4 mg/Kg。用六种实验日粮中的一种饲喂育雏鸡 90 天。在以 PP 为基础的饮食中补充 2-4 mg n.Se/Kg 可显着提高孵化后 3 天幼虫 (3DPH) 幼虫的存活率以及新生和 3DPH 幼虫的全身长度。血清三碘甲状腺原氨酸（T ₃) 以 1-4 mg n.Se/kg 的 PP 为基础的饮食显着降低了女性，同时，喂食 1-4 mg n.Se/kg 的 PP 饮食的女性血清甲状腺素 (T ₄ ) 增加。以 FM 为基础的饮食喂养的女性血清 T ₃水平低于以 PP 为基础的饮食但未补充 n.Se 的女性（0.6 vs. 1.9 nmol/L ^-1）。同时，饲喂以 FM 为基础的日粮的亲鱼的血清 T ₄明显高于饲喂以 PP 为主的日粮的亲鱼（21.9对14.3 nmol/L ^-1）。T ₃和 T _4的水平雌性 3DPH 幼虫在未添加 n.Se 的 PP 饲料组中低于 FM 和 n.Se 补充 PP 组（P  < 0.05）。随着 PP 饮食中 n.Se 水平的增加，女性的血清碱性磷酸酶 (ALP) 增加。最高和最低的 ALP 水平分别来自饲喂未添加 n.Se 的 PP 基饲料的雌性和饲喂 1 mg n.Se/Kg PP 基饲料的雌性的 3DPH 幼虫（P  < 0.05）。本研究的结果表明，在以 PP 为基础的饮食中补充 2-4 mg n.Se 可以提高A. arabicus育雏器和幼虫的性能，这与该物种甲状腺激素的调节有关。