Triple-Probe DNA Framework-Based Transistor for SARS-CoV-2 10-in-1 Pooled Testing,Nano Letters

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Triple-Probe DNA Framework-Based Transistor for SARS-CoV-2 10-in-1 Pooled Testing
Nano Letters ( IF 9.6 ) Pub Date : 2022-04-15 , DOI: 10.1021/acs.nanolett.2c00415
Yungen Wu _{1,

2} , Daizong Ji _{1,

2} , Changhao Dai _{1,

2} , Derong Kong _{1,

2} , Yiheng Chen _{1,

2} , Liqian Wang _{1,

2} , Mingquan Guo ₃ , Yunqi Liu ₁ , Dacheng Wei _{1,

2}

Affiliation

Accurate and population-scale screening technology is crucial in the control and prevention of COVID-19, such as pooled testing with high overall testing efficiency. Nevertheless, pooled testing faces challenges in sensitivity and specificity due to diluted targets and increased contaminations. Here, we develop a graphene field-effect transistor sensor modified with triple-probe tetrahedral DNA framework (TDF) dimers for 10-in-1 pooled testing of SARS-CoV-2 RNA. The synergy effect of triple probes as well as the special nanostructure achieve a higher binding affinity, faster response, and better specificity. The detectable concentration reaches 0.025–0.05 copy μL^–1 in unamplified samples, lower than that of the reverse transcript-polymerase chain reaction. Without a requirement of nucleic-acid amplification, the sensors identify all of the 14 positive cases in 30 nasopharyngeal swabs within an average diagnosis time of 74 s. Unamplified 10-in-1 pooled testing enabled by the triple-probe TDF dimer sensor has great potential in the screening of COVID-19 and other epidemic diseases.

中文翻译：

用于 SARS-CoV-2 10 合 1 联合测试的基于三探针 DNA 框架的晶体管

准确的人群规模筛查技术对于控制和预防 COVID-19 至关重要，例如总体检测效率较高的汇总检测。然而，由于靶标稀释和污染增加，混合检测在灵敏度和特异性方面面临挑战。在这里，我们开发了一种经过三探针四面体 DNA 框架 (TDF) 二聚体修饰的石墨烯场效应晶体管传感器，用于 SARS-CoV-2 RNA 的 10 合 1 混合测试。三重探针的协同作用以及特殊的纳米结构实现了更高的结合亲和力、更快的响应和更好的特异性。未扩增样品中的可检测浓度达到0.025-0.05 copy μL ^–1，低于逆转录聚合酶链式反应的检测浓度。无需核酸扩增，传感器即可在平均74秒的诊断时间内识别出30份鼻咽拭子中的所有14例阳性病例。由三探针 TDF 二聚体传感器实现的非放大 10 合 1 联合检测在筛查 COVID-19 和其他流行病方面具有巨大潜力。

更新日期：2022-04-15

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