Insect pests and weeds are the two major biotic factors affecting crop yield in the modern agricultural system. In this study, a brown planthopper (BPH) resistance gene (BPH9) and glufosinate tolerance gene (bar) were stacked into a single T-DNA cassette and transformed into an indica rice (Oryza sativa L.) line Guangzhan 63-4S. A stable transgenic line H23 with a single T-DNA insert was generated, with the T-DNA cassette located on chromosome 3. Field resistance trial using H23 revealed high tolerance to glufosinate and excellent resistance to BPH. These results propose H23 as valuable germplasm for BPH-resistance and glufosinate-tolerance breeding in rice.

虫害和杂草是现代农业系统中影响作物产量的两大生物因素。在这项研究中，将褐飞虱（BPH）抗性基因（BPH9）和草铵膦耐受性基因（bar）堆叠到单个T-DNA盒中，并转化到籼稻（Oryza sativa L.）系广展63-4S中。产生了具有单个 T-DNA 插入片段的稳定转基因品系 H23，T-DNA 盒位于 3 号染色体上。使用 H23 的田间抗性试验显示出对草铵膦的高耐受性和对 BPH 的优异抗性。这些结果表明 H23 作为水稻抗褐飞虱和耐草铵膦育种的宝贵种质。