Abstract

The present study evaluated the effect of high-fluoride dentifrice on dentine demineralization and bacterial composition in a multispecies biofilm model in vitro. A seven-organism bacterial consortium was grown on bovine dentine discs in a high-throughput active attachment model. The biofilms were submitted twice per day to the following dentifrices treatments: 5,000 ppm F, 1,100 ppm F, with placebo as a negative control. After 5 days of biofilm growth, dentine samples were assessed by transversal microradiography, the biofilm was collected for bacterial counts and the pH of the media was determined. Lower integrated mineral loss values were observed when 5,000 ppm F-treatment was used compared to the other treatments. Overall microbiological counts decreased with increasing F-concentration as well the pH of the media throughout the experiment. The 5,000 ppm F-treatment caused a shift in microbial composition and reduced dentine demineralization in the in-vitro experimental model.

摘要

本研究在体外多物种生物膜模型中评估了高氟牙膏对牙本质脱矿质和细菌组成的影响。. 在高通量主动附着模型中，在牛牙本质盘上生长了一个七生物细菌聚生体。生物膜每天两次接受以下牙膏处理：5,000 ppm F、1,100 ppm F，安慰剂作为阴性对照。生物膜生长 5 天后，通过横向显微射线照相评估牙本质样品，收集生物膜用于细菌计数并确定培养基的 pH 值。与其他处理相比，当使用 5,000 ppm F 处理时，观察到较低的综合矿物质损失值。在整个实验过程中，随着 F 浓度以及培养基 pH 值的增加，总体微生物计数降低。5,000 ppm F 处理导致微生物组成发生变化并减少体外牙本质脱矿质实验模型。