In this study, efficient transmission ways of ‘Ca. P. pyri’ which causes Pear Decline (PD) disease and response of different rootstock-scion combinations to this pathogen were evaluated. For graft transmission trials, fifty BA29 clonal rootstocks were grafted with buds taken from a ‘Ca. P. pyri’ infected pear tree, cv. Deveci, and the transmission rate was found to be 8% according to PCR/RFLP analyses. Growth retardation was detected in some grafted plants but the specific reddening symptoms for PD were not observed during the 2 years of observation. Cacopsylla pyri L., playing important role for the transmission of pear decline phytoplasma in open field, was used for experimental transmission trials. It has been shown that it can acquire phytoplasma (in 1 day) and transmit it (in 2 weeks to healthy pear saplings). Therefore it was revealed that C. pyri plays an important role in pear decline epidemiology. When the response of several rootstock-scion combinations to ‘Ca. P. pyri’was evaluated over two vegetative periods by visual monitoring of symptom development and by PCR analyses, two Santa Maria and one Williams plants grafted on OHF333 and one Deveci plant grafted on P. communis were found infected by ‘Ca. P. pyri’, but no infection was detected in a local cv. Ankara grafted on any rootstocks. Among the commercial cultivars, our local cv. Deveci was found the most sensitive and cv. Ankara was the most tolerant. The use of healthy plant materials, as well as the appropriate control of the vector will play an important role in disease control.

在本研究中，' Ca.的有效传播途径。评估了导致梨衰退 (PD) 病的 P. pyri' 以及不同砧木-接穗组合对该病原体的反应。对于嫁接传播试验，五十个 BA29 克隆砧木与取自 'Ca. P. pyri' 感染的梨树，简历。Deveci，根据 PCR/RFLP 分析发现传播率为 8%。在一些嫁接植物中检测到生长迟缓，但在 2 年的观察期间未观察到特定的 PD 发红症状。梨果蝇L.对梨衰退植原体在露地传播中起重要作用，用于实验传播试验。已经表明，它可以获取植原体（在 1 天内）并将其传播（在 2 周内传播给健康的梨树苗）。因此揭示了C. pyri在梨衰退流行病学中起重要作用。当几种砧木接穗组合对'Ca。P. pyri'通过症状发展的视觉监测和 PCR 分析在两个营养期进行了评估，发现嫁接在 OHF333 上的两株 Santa Maria 和一株威廉姆斯植物以及嫁接在 P.communis 上的一株 Deveci 植物被' Ca。P. pyri'，但在本地 cv 中未检测到感染。安卡拉嫁接在任何砧木上。在商业品种中，我们当地的品种。Deveci 被发现是最敏感的和 cv。安卡拉是最宽容的。使用健康的植物材料，以及对病媒进行适当的控制，将在疾病控制中发挥重要作用。