In this study, the dietary effect of garlic powder (GP) as an herbal supplement on the growth performance, digestive enzymes, innate immunity, and antioxidant capacity of narrow-clawed crayfish, Postantacus leptodactylus, was investigated. In a 90-day feeding trial, the crayfish (N = 360; 25.90 ± 0.41 g initial weight) were fed with a basal diet (control; GP-free) and supplemented diets with three levels of GP (0.5%, 1%, and 2%). The highest final weight and specific growth rate were obtained in the crayfish fed with GP at 1% and 2% (P < 0.05) and the weight gain in GP 2% treatment was 1.6 fold more than the control group. Also, the feed conversion rate and protein efficiency rate were improved in the GP-add groups compared to the control group (P < 0.05). Furthermore, the highest counts of total hemocyte, large-granular cell, and semi-granular cell were recorded in those fed with GP 2% diet. The crayfish fed GP 2% diet showed the highest and lowest body crude lipid (0.24 ± 0.03%) and protein contents (15.95 ± 0.12%), respectively. The highest protease (1.92 ± 0.03 U/ml) and amylase (8.36 ± 0.3 U/ml) activities were detected in the crayfish hepatopancreas fed with GP at 2% (P < 0.05). The highest lysozyme activity (21.26 ± 2.08 U/ml) was observed in the group fed with GP 2% diet, which showed a significant difference from others. Besides, total plasma protein and phenoloxidase activity in the groups fed with GP 1% and GP 2% diets were significantly higher than the control group. Moreover, the activities of superoxide dismutase (79.30 ± 2.19 U/ml) and nitric oxide synthase (4.16 ± 0.05 U/ml) in the plasma were significantly increased in GP 2% diet with respect to the GP 0.5% and control groups (P < 0.05). These findings confirmed the beneficial effects of dietary GP, especially at 2%, on growth rate, physiological performance, innate immunity, and antioxidant capacity in P. leptodactylus.

在这项研究中，研究了大蒜粉 (GP) 作为草药补充剂对窄爪小龙虾Postantacus leptodactylus生长性能、消化酶、先天免疫和抗氧化能力的影响。在为期 90 天的喂养试验中，小龙虾（N = 360；25.90 ± 0.41 g 初始体重）饲喂基础日粮（对照；无 GP）和添加三种水平 GP（0.5%、1%、和 2%）。以 1% 和 2% 的 GP 喂养的小龙虾终重和比生长率最高（P  < 0.05），且 2% 的 GP 处理的增重是对照组的 1.6 倍。此外，与对照组相比，GP-add 组的饲料转化率和蛋白质效率都有所提高（P < 0.05)。此外，总血细胞、大颗粒细胞和半颗粒细胞的最高计数记录在饲喂 GP 2% 饮食的组中。饲喂 2% GP 日粮的小龙虾的身体粗脂（0.24 ± 0.03%）和蛋白质含量（15.95 ± 0.12%）分别最高和最低。在 2% 的 GP 喂养的小龙虾肝胰腺中检测到最高的蛋白酶 (1.92 ± 0.03 U/ml) 和淀粉酶 (8.36 ± 0.3 U/ml) 活性 ( P < 0.05)。GP 2%日粮组溶菌酶活性最高（21.26±2.08 U/ml），与其他组有显着差异。此外，饲喂GP 1%和GP 2%日粮组的总血浆蛋白和酚氧化酶活性显着高于对照组。此外，与 GP 0.5% 和对照组相比，GP 2% 饮食中血浆中超氧化物歧化酶 (79.30 ± 2.19 U/ml) 和一氧化氮合酶 (4.16 ± 0.05 U/ml) 的活性显着增加（P  < 0.05)。这些发现证实了日粮 GP（尤其是 2% 的 GP）对P. leptodactylus的生长速度、生理机能、先天免疫和抗氧化能力的有益影响。