Polyhydroxyalkanoates (PHA) are a family of biodegradable and biocompatible plastics with potential to replace petroleum based plastics. Diversity of PHA monomer structures provides flexibility in material properties to suit more applications. In this study, 5-hydroxyvalerate (5HV) synthesis pathway was established based on intrinsic alcohol/aldehyde dehydrogenases. The PHA polymerase cloned from Cupriavidus necator functions to polymerize 5HV into its copolymers in ratios ranging from 8% to 32%. Elastic copolymer P(85% 3HB-co-15% 5HV) was generated with an elongation at break and a Young's modulus of 1283% and 73.1 MPa, respectively. The recombinant H. bluephagenesis was able to convert various diols including 1, 3-propanediol, 1, 4-butanediol and 1, 5-pentanediol into PHA, leading to 13 PHA polymers including transparent P(53% 3HB-co-20% 4HB-co-27% 5HV) and sticky P(3HB-co-3HP-co-4HB-co-5HV). The engineered H. bluephagenesis was successfully grown in a 7-L bioreactor to produce the highly elastic P(85% 3HB-co-15% 5HV) and the sticky P(3HB-co-3HP-co-4HB-co-5HV), demonstrating their potential for industrial scale-up.

聚羟基链烷酸酯 (PHA) 是一类可生物降解和生物相容的塑料，具有替代石油基塑料的潜力。PHA 单体结构的多样性为材料特性提供了灵活性，以适应更多应用。在本研究中，基于内在醇/醛脱氢酶建立了 5-羟基戊酸 (5HV) 合成途径。从Cupriavidus necator克隆的 PHA 聚合酶的功能是将 5HV 聚合成其共聚物，其比例范围为 8% 至 32%。生成的弹性共聚物 P(85% 3HB - co -15% 5HV) 的断裂伸长率和杨氏模量分别为 1283% 和 73.1 MPa。重组H. bluephagenesis能够将包括 1, 3-丙二醇、1, 4-丁二醇和 1, 5-戊二醇在内的各种二醇转化为 PHA，产生 13 种 PHA 聚合物，包括透明 P(53% 3HB- co -20% 4HB- co -27% 5HV) 和粘性 P(3HB- co -3HP- co -4HB- co -5HV)。工程化的H. bluephagenesis在 7-L 生物反应器中成功生长，以产生高弹性 P(85% 3HB- co -15% 5HV) 和粘性 P(3HB- co -3HP- co -4HB- co -5HV) ，展示了它们在工业规模化方面的潜力。