Cancer is now responsible for the major leading cause of death worldwide. It is noteworthy that lung cancer has been recognised as the highest incidence (11.6%) and mortality (18.4%) for combined sexes among a variety of cancer diseases. Therefore, it is of great value to investigate the mechanical properties of lung cancerous cells for early diagnosis. This paper focus on the influence of measurement parameters on the measured central Young's moduli of single live A549 cell in vitro based on the force spectroscopy mode of atomic force microscopy (AFM). The effects of the measurement parameters on the measured central Young's moduli were analysed by fitting the force–depth curves utilising the Sneddon model. The results revealed that the Young's moduli of A549 cells increased with the larger indentation force, higher indentation speed, less retraction time, deeper Z length and lower purity percentage of serum. The Young's moduli of cells increased first and then decreased with the increasing dwell time. Hence, this research may have potential significance to provide reference for the standardised detection of a single cancerous cell in vitro using AFM methodologies.

中文翻译：

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测量参数对体外原子力显微镜表征癌细胞核的影响

癌症现在是全球主要的主要死因。值得注意的是，肺癌已被公认为多种癌症疾病中男女混合发病率最高（11.6%）和死亡率最高（18.4%）的癌症。因此，研究肺癌细胞的力学特性对早期诊断具有重要价值。本文基于原子力显微镜（AFM）的力谱模式，重点研究了测量参数对体外单活A549细胞中心杨氏模量测量的影响。通过使用 Sneddon 模型拟合力-深度曲线来分析测量参数对测量的中心杨氏模量的影响。结果表明，A549细胞的杨氏模量随着压入力越大、压入速度越高、更短的回缩时间，更深的Z长度和更低的血清纯度百分比。细胞的杨氏模量随着停留时间的增加先增加后减少。因此，该研究可能具有潜在的意义，为使用AFM方法在体外标准化检测单个癌细胞提供参考。