A sensitive electrochemical sensor for the detection of Pb²⁺ was developed based on PtNPs@Cu-MOF signal probe and DNA walker signal amplification. The electrochemical sensor can be used for the detection of Pb²⁺ based on the recognition of rA site in DNA walker-Substrate Strand (SS). The SS mixture is assembled on the AuE surface through the Au-S bond and can split and form two single chains S1 and S2 for the DNA walker reaction. Most importantly, The DNA walker can move automatically and participate in the next part cycle for the signal amplification. Furthermore, for an excellent electrochemical signal PtNPs@Cu-MOF nanocomposite was introduced in electrochemical sensor based on the effect between S1 chain and HP hairpin structure. This nanocomposite can provide a larger specific surface, adhesion sites and excellent electrical conductivity for the sensor. This sensitive electrochemical sensor showed that the concentration of Pb²⁺ presented a linear correlation range from 3 × 10⁻¹² to 5 × 10⁻⁶ mol/L and the limit of detection (LOD) was 0.2 pmol/L. The designed sensor has a good recovery in two real samples of tap water and orange juice, indicating it can be used as an alternative detection method via comparison with other detection method. The sensor displayed a promising possibility in actual monitoring.

基于PtNPs@Cu-MOF信号探针和DNA walker信号放大技术开发了一种检测Pb ²⁺的灵敏电化学传感器。电化学传感器可用于检测Pb ²⁺基于对 DNA walker-Substrate Strand (SS) 中 rA 位点的识别。SS混合物通过Au-S键组装在AuE表面，可以分裂形成两条单链S1和S2，用于DNA walker反应。最重要的是，DNA walker 可以自动移动并参与下一部分循环进行信号放大。此外，为了获得优异的电化学信号，基于 S1 链和 HP 发夹结构之间的影响，将 PtNPs@Cu-MOF 纳米复合材料引入电化学传感器中。这种纳米复合材料可以为传感器提供更大的比表面、粘附位点和出色的导电性。这种灵敏的电化学传感器表明 Pb ^{2+的浓度呈现出从 3 × 10 -12}的线性相关范围至5×10 ^-6  mol/L，检测限(LOD)为0.2 pmol/L。所设计的传感器在自来水和橙汁两个真实样品中具有良好的回收率，通过与其他检测方法的比较，表明它可以作为替代检测方法。该传感器在实际监测中显示出有希望的可能性。