Intensive aquaculture supports higher production per unit of area, but it may be associated by stress and health problems in fish. Thus, researches are continuing to find applicable methods for suppressing such negative effects. Dietary supplementation with medicinal plant is known as a promising method in this field. Accordingly, the present study aimed at assessing the benefits of dietary Artemisia, Artemisia annua, supplementation (0, 2.5, 5 and 10 g/kg) on growth performance, stress, antioxidant and immunological responses of common carp, Cyprinus carpio, held at a high stocking density (10 g/L vs. 3 g/L) for 70 days. Increase in fish stocking density significantly (p < 0.05) reduced growth performance, haematological parameters (red blood cell, haematocrit, haemoglobin, leukocyte and lymphocyte) and immune responses (total protein, complement and total immunoglobulin), but increased neutrophil, lysozyme, stress responses (cortisol, glucose, aspartate aminotransferase and lactate dehydrogenase), antioxidant enzymes’ activities (superoxide dismutase, catalase and glutathione peroxidase) and lipid peroxidation. Artemisia supplementation significantly (p < 0.05) restored haematological parameters, leukocyte count and mitigated the changes in lymphocyte, neutrophil, stress and immunological parameters, antioxidant enzymes’ activities and lipid peroxidation. Based on the results, dietary Artemisia supplementation is not capable to restore growth retardation of common carp under high stocking density; however, it mitigates the adverse effects of high stocking density on haematological, antioxidant and immunological parameters. Supplementation at 5 g/kg is recommended for carp feed formulation.

中文翻译：

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日粮青蒿、青蒿、补充剂可提高高放养密度下鲤鱼的福利

集约化水产养殖支持更高的单位面积产量，但这可能与鱼类的压力和健康问题有关。因此，研究正在继续寻找抑制这种负面影响的适用方法。膳食补充剂与药用植物被认为是该领域有前途的方法。因此，本研究旨在评估日粮蒿、青蒿、补充剂（0、2.5、5 和 10 g/kg）对鲤鱼鲤鱼的生长性能、应激、抗氧化和免疫反应的益处。 70 天的高放养密度（10 g/L 对 3 g/L）。鱼类放养密度显着增加 ( p < 0.05) 降低生长性能、血液学参数（红细胞、血细胞比容、血红蛋白、白细胞和淋巴细胞）和免疫反应（总蛋白、补体和总免疫球蛋白），但增加中性粒细胞、溶菌酶、应激反应（皮质醇、葡萄糖、天冬氨酸氨基转移酶）和乳酸脱氢酶）、抗氧化酶的活性（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）和脂质过氧化。艾草增补显着 ( p < 0.05) 恢复血液学参数、白细胞计数并减轻淋巴细胞、中性粒细胞、应激和免疫学参数、抗氧化酶活性和脂质过氧化的变化。结果表明，日粮中添加青蒿不能恢复鲤鱼在高放养密度下的生长迟缓；然而，它减轻了高饲养密度对血液学、抗氧化和免疫学参数的不利影响。鲤鱼饲料配方推荐添加量为 5 g/kg。