Review and Meta-Analysis: SARS-CoV-2 and Enveloped Virus Detection in Feces and Wastewater,ChemBioEng Reviews

当前位置： X-MOL 学术 › ChemBioEng Rev. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Review and Meta-Analysis: SARS-CoV-2 and Enveloped Virus Detection in Feces and Wastewater
ChemBioEng Reviews ( IF 6.2 ) Pub Date : 2022-03-03 , DOI: 10.1002/cben.202100039
Charlotte Twigg ₁ , Jannis Wenk ₁

Affiliation

Detection and quantification of viruses supplies key information on their spread and allows risk assessment for public health. In wastewater, existing detection methods have been focusing on non-enveloped enteric viruses due to enveloped virus transmission, such as coronaviruses, by the fecal-oral route being less likely. Since the beginning of the SARS-CoV-2 pandemic, interest and importance of enveloped virus detection in wastewater has increased. Here, quantitative studies on SARS-CoV-2 occurrence in feces and raw wastewater and other enveloped viruses via quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) during the early stage of the pandemic until April 2021 are reviewed, including statistical evaluation of the positive detection rate and efficiency throughout the detection process involving concentration, extraction, and amplification stages. Optimized and aligned sampling protocols and concentration methods for enveloped viruses, along with SARS-CoV-2 surrogates, in wastewater environments may improve low and variable recovery rates providing increased detection efficiency and comparable data on viral load measured across different studies.

中文翻译：

回顾和荟萃分析：粪便和废水中的 SARS-CoV-2 和包膜病毒检测

病毒的检测和量化提供了有关其传播的关键信息，并允许对公共卫生进行风险评估。在废水中，现有的检测方法一直专注于非包膜肠道病毒，因为包膜病毒通过粪口途径传播的可能性较小，例如冠状病毒。自 SARS-CoV-2 大流行开始以来，废水中包膜病毒检测的兴趣和重要性不断增加。本文回顾了大流行早期至 2021 年 4 月期间通过定量实时逆转录聚合酶链反应 (RT-qPCR) 在粪便和生废水和其他包膜病毒中发生 SARS-CoV-2 的定量研究，包括统计在涉及浓度的整个检测过程中评估阳性检测率和效率，提取和放大阶段。废水环境中包膜病毒以及 SARS-CoV-2 替代物的优化和对齐采样协议和浓缩方法可能会提高低和可变的回收率，从而提高检测效率和在不同研究中测量的病毒载量的可比数据。

更新日期：2022-03-03

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文