The abuse of antibiotics in aquaculture has led to the increasing rate of antibiotic resistance of aquatic bacteria including Aeromonas, which is an increasing threat to environmental and human health. To date, no epidemiological cut-off values (CO_WT) for Aeromonas spp. have been established by the Clinical and Laboratory Standards Institute nor the European Commission on Antimicrobial Susceptibility Testing. In this study, commercially prepared minimum inhibitory concentration (MIC) test 96-well plates (dry-form plates) were used to determine the MIC of eight antimicrobial agents against 556 Aeromonas strains. The obtained MIC distributions were simulated and analyzed by NRI and ECOFFinder to obtain tentative CO_WT values for Aeromonas spp. The CO_WT values of eight kinds of representative antimicrobial agents including trimethoprim–sulfamethoxazole, erythromycin, doxycycline, neomycin, colistin, florfenicol, enrofloxacin, and ceftazidime for Aeromonas spp. were established and were 0.25, 64/32, 4/2, 8, 4, 1, 0.062/0.125, and 0.5 μg/mL, respectively. Results showed that Aeromonas spp. had a very high proportion of non-wild-type strains to enrofloxacin, florfenicol, and doxycycline, which are the most widely used antimicrobials in aquaculture. The CO_WT values for Aeromonas spp. obtained in this study can contribute to the final establishment of CO_WT for Aeromonas spp. internationally.

中文翻译：

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水生气单胞菌对八种抗菌药物的流行病学耐药临界值的建立

水产养殖中抗生素的滥用导致包括气单胞菌在内的水生细菌的抗生素耐药率增加，这对环境和人类健康构成越来越大的威胁。迄今为止，没有气单胞菌属的流行病学临界值 (CO _WT ) 。由临床和实验室标准协会或欧洲抗菌药物敏感性测试委员会建立。在这项研究中，商业制备的最低抑菌浓度 (MIC) 测试 96 孔板（干板）用于确定八种抗菌剂对 556种气单胞菌菌株的 MIC。通过 NRI 和 ECOFFinder 对获得的 MIC 分布进行模拟和分析，以获得暂定的 CO _WT气单胞菌属的值。甲氧苄啶-磺胺甲恶唑、红霉素、强力霉素、新霉素、粘菌素、氟苯尼考、恩诺沙星、头孢他啶等8种代表性抗菌药物的CO _WT值。分别为 0.25、64/32、4/2、8、4、1、0.062/0.125 和 0.5 μg/mL。结果表明，气单胞菌属。水产养殖中使用最广泛的抗菌药物恩诺沙星、氟苯尼考和强力霉素的非野生型菌株比例非常高。气单胞菌属的 CO _WT值。_{本研究中获得的 CO WT}有助于最终建立气单胞菌属 国际上。