Dietary supplementation with a fucoidan-rich Ascophyllum nodosum extract (ANE), possessing an in vitro anti-Salmonella Typhimurium activity could be a promising on-farm strategy to control Salmonella infection in pigs. The objectives of this study were to: 1) evaluate the anti-S. Typhimurium activity of ANE (containing 46.6% fucoidan, 18.6% laminarin, 10.7% mannitol, 4.6% alginate) in vitro, and; 2) compare the effects of dietary supplementation with ANE and Zinc oxide (ZnO) on growth performance, Salmonella shedding and selected gut parameters in naturally infected pigs. This was established post-weaning (newly weaned pig experiment) and following regrouping of post-weaned pigs and experimental re-infection with S. Typhimurium (challenge experiment). In the in vitro assay, increasing ANE concentrations led to a linear reduction in S. Typhimurium counts (P < 0.05). In the newly weaned pig experiment (12 replicates/treatment), high ANE supplementation increased gain to feed ratio, similar to ZnO supplementation, and reduced faecal Salmonella counts on d 21 compared to the low ANE and control groups (P < 0.05). The challenge experiment included thirty-six pigs from the previous experiment that remained on their original dietary treatments (control and high ANE groups with the latter being renamed to ANE group) apart from the ZnO group which transitioned onto a control diet on d 21 (ZnO-residual group). These dietary treatments had no effect on performance, faecal scores, Salmonella shedding or colonic and caecal Salmonella counts (P > 0.05). ANE supplementation decreased the Enterobacteriaceae counts compared to the control. Enterobacteriaceae counts were also reduced in the ZnO-residual group compared to the control (P < 0.05). ANE supplementation decreased the expression of interleukin 22 and transforming growth factor beta 1 in the ileum compared to the control (P < 0.05). ANE supplementation was associated with some beneficial changes in the composition of the colonic microbiota, Salmonella shedding, and the expression of inflammatory genes associated with persistent Salmonella infection.

中文翻译：

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富含岩藻依聚糖的结节藻提取物在减少自然感染沙门氏菌的断奶猪的沙门氏菌脱落和改善胃肠道健康方面的潜力

日粮中添加富含岩藻依聚糖的结节藻提取物 (ANE)，具有体外抗鼠伤寒沙门氏菌活性，可能是控制猪沙门氏菌感染的一种有前景的农场策略。本研究的目的是：1）评估抗 S。ANE（含有 46.6% 岩藻依聚糖、18.6% 海带多糖、10.7% 甘露醇、4.6% 海藻酸盐）的鼠伤寒活性，以及​​；2) 比较添加 ANE 和氧化锌 (ZnO) 的日粮对自然感染猪的生长性能、沙门氏菌脱落和选定肠道参数的影响。这是在断奶后（新断奶猪实验）和断奶后猪重新分组和用鼠伤寒沙门氏菌再次感染实验（挑战实验）后建立的。在体外测定中，增加 ANE 浓度导致 S. 鼠伤寒菌计数 (P < 0.05)。在新断奶猪实验中（12 次重复/处理），与低 ANE 组和对照组相比，高 ANE 补充增加了增重比，类似于 ZnO 补充，并且在第 21 天减少了粪便沙门氏菌计数（P < 0.05）。挑战实验包括来自先前实验的 36 头猪，除了 ZnO 组在第 21 天过渡到对照饮食（ZnO -残差组）。这些饮食治疗对性能、粪便评分、沙门氏菌脱落或结肠和盲肠沙门氏菌计数没有影响（P > 0.05）。与对照相比，ANE 补充剂降低了肠杆菌科细菌的数量。与对照组相比，ZnO 残留组的肠杆菌科细菌数量也减少了（P < 0.05）。与对照组相比，ANE 补充剂降低了回肠中白细胞介素 22 和转化生长因子 β1 的表达（P < 0.05）。ANE 补充剂与结肠微生物群组成、沙门氏菌脱落和与持续性沙门氏菌感染相关的炎症基因表达的一些有益变化有关。