Pseudomonas aeruginosa post-translational responses to elevated c-di-GMP levels,Molecular Microbiology

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Pseudomonas aeruginosa post-translational responses to elevated c-di-GMP levels
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-04-01 , DOI: 10.1111/mmi.14902
Sarina Bense _{1,

2} , Julius Witte _{2,

3} , Matthias Preuße ₁ , Michal Koska _{1,

2} , Lorena Pezoldt _{1,

2} , Astrid Dröge ₁ , Oliver Hartmann _{1,

2} , Mathias Müsken ₄ , Julia Schulze ₅ , Timm Fiebig ₅ , Heike Bähre ₆ , Sebastian Felgner _{1,

2} , Andreas Pich ₃ , Susanne Häussler _{1,

2,

7,

8}

Affiliation

C-di-GMP signaling can directly influence bacterial behavior by affecting the functionality of c-di-GMP-binding proteins. In addition, c-di-GMP can exert a global effect on gene transcription or translation, for example, via riboswitches or by binding to transcription factors. In this study, we investigated the effects of changes in intracellular c-di-GMP levels on gene expression and protein production in the opportunistic pathogen Pseudomonas aeruginosa. We induced c-di-GMP production via an ectopically introduced diguanylate cyclase and recorded the transcriptional, translational as well as proteomic profile of the cells. We demonstrate that rising levels of c-di-GMP under growth conditions otherwise characterized by low c-di-GMP levels caused a switch to a non-motile, auto-aggregative P. aeruginosa phenotype. This phenotypic switch became apparent before any c-di-GMP-dependent role on transcription, translation, or protein abundance was observed. Our results suggest that rising global c-di-GMP pools first affects the motility phenotype of P. aeruginosa by altering protein functionality and only then global gene transcription.

中文翻译：

铜绿假单胞菌对 c-di-GMP 水平升高的翻译后反应

C-di-GMP 信号可以通过影响 c-di-GMP 结合蛋白的功能直接影响细菌行为。此外，c-di-GMP 可以对基因转录或翻译产生全局影响，例如，通过核糖开关或通过与转录因子结合。在这项研究中，我们研究了细胞内 c-di-GMP 水平的变化对机会性病原体铜绿假单胞菌中基因表达和蛋白质产生的影响。我们通过异位引入的二鸟苷酸环化酶诱导 c-di-GMP 产生，并记录了细胞的转录、翻译和蛋白质组学特征。我们证明，在以低 c-di-GMP 水平为特征的生长条件下，c-di-GMP 水平的升高导致了向非运动、自动聚集的转变铜绿假单胞菌表型。在观察到任何 c-di-GMP 依赖于转录、翻译或蛋白质丰度的作用之前，这种表型转换就变得很明显。我们的研究结果表明，不断上升的全球 c-di-GMP 池首先通过改变蛋白质功能和全局基因转录来影响铜绿假单胞菌的运动表型。

更新日期：2022-04-01

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