As a critical factor in the sustainability of fisheries, gynogenesis can be used to provide a shortcut for the genetic improvement of the endangered and degraded germplasm resources of the important Yellow River fish Silurus lanzhouensis. In this study, ultraviolet-inactivated yellow catfish Pelteobagrus fulvidraco sperm were used to induce meiotic gynogenesis in S. lanzhouensis through cold shock, and the optimal conditions for the genetic inactivation of the sperm and cold shock treatment were selected. Using microsatellite markers and gonadal histology, the genetic purity and sex determination of the gynogenetic offspring of S. lanzhouensis were analysed. The optimal induction conditions were as follows: treatment with P. fulvidraco sperm with 107- µW/cm² ultraviolet irradiation for 30 min, fertilization of S. lanzhouensis eggs for 6 min and subsequent cold shock treatment at 4–6°C for 25 min. Flow cytometry and microsatellite markers confirmed the successful induction of meiogynogenesis. The homozygosity of the gynogenetic group (0.63) was higher than that of the normal control group (0.32). The sex ratio was determined through gonad histological observations, and 100% of the offspring in the gynogenetic groups were female, strongly indicating that the sex determination system of S. lanzhouensis is the XX/XY-type. In this study, inactivated heterologous sperm were used to successfully obtain meiogynogenetic individuals of S. lanzhouensis, and the study provides an important basis for the establishment of a pure line of these endangered fish and sex-controlled breeding of S. lanzhouensis.

中文翻译：

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异源精子对兰州鲶鱼（Silurus lanzhouensis）的减数分裂雌核发育的诱导及雌性同配性的证据

作为渔业可持续发展的关键因素，雌核发育可以为黄河重要鱼类兰州鲶濒危退化种质资源的遗传改良提供一条捷径。在这项研究中，紫外线灭活的黄鲶鱼黄颡鱼精子被用来诱导S的减数分裂雌核发育。 通过冷休克，选择了精子基因失活和冷休克处理的最佳条件。使用微卫星标记和性腺组织学，对S的雌核发育后代的遗传纯度和性别决定。 兰州进行了分析。最佳诱导条件如下：用P处理。 fulvidraco精子用 107-μW/cm ²紫外线照射 30 分钟，使S受精。 兰州虫卵 6 分钟，随后在 4-6°C 冷激处理 25 分钟。流式细胞术和微卫星标记证实了成功诱导减数分裂。雌核发育组的纯合度（0.63）高于正常对照组（0.32）。性别比是通过性腺组织学观察确定的，雌核发育组100%的后代为雌性，强烈表明S的性别决定系统。 兰州是 XX/XY 型。在这项研究中，灭活的异源精子被用于成功获得S的减数分裂个体。 该研究为建立这些濒危鱼类的纯种系和S 的性别控制育种提供了重要依据。 兰州_