Biological preparation and characterization of surfactant-like peptides,Journal of Surfactants and Detergents

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Biological preparation and characterization of surfactant-like peptides
Journal of Surfactants and Detergents ( IF 1.6 ) Pub Date : 2022-03-29 , DOI: 10.1002/jsde.12590
Shujuan Meng ₁ , Yadong Wang ₁ , Jing Mu ₁ , Zhengjun Pang ₁ , Fenghuan Wang ₁ , Yonghong Liao ₁

Affiliation

Surfactant-like peptides are a class of small molecule short peptides with surface activity designed independently by imitating the structural characteristics of natural lipid molecules. Surfactant-like peptides have the advantages of no pollution to the environment, easy biodegradation, strong bacteriostasis, and nutrition, which accords with the development concept of the modern green industry. They are widely used in many fields such as cleaning, food, cosmetics, medicine. At present, such surfactants are mainly synthesized by expensive chemical methods. The efficient synthesis of surfactant-like peptides by biological methods has become a significant research direction. This method is environmentally friendly and can achieve low-cost and efficient production. In this study, three novel surfactant-like peptides were designed and synthesized by biological methods and successfully expressed in vitro. By optimizing of the expression host, expression vector, and fermentation conditions, the optimal expression amount was finally obtained. In addition, these surfactant-like peptides were found to have good surface activity and low critical micelle concentration. More importantly, they are pH-responsive and can self-assemble to form nanostructures in different solutions, which provides essential research value for the production of novel nanomaterials in the future. This study is the first to construct and express three novel surfactant-like peptides by biological methods, which effectively reduce the production cost compared to chemical methods; and it was also found to have excellent surfactant properties. This provides the basis for large-scale industrial production and application.

中文翻译：

表面活性剂样肽的生物制备及表征

表面活性剂样肽是通过模仿天然脂质分子的结构特征自主设计的一类具有表面活性的小分子短肽。类表面活性剂肽具有对环境无污染、易生物降解、抑菌性强、营养丰富等优点，符合现代绿色产业的发展理念。广泛应用于清洁、食品、化妆品、医药等诸多领域。目前，此类表面活性剂主要通过昂贵的化学方法合成。通过生物方法高效合成表面活性剂样肽已成为重要的研究方向。该方法对环境友好，可实现低成本高效生产。在这项研究中，通过生物学方法设计合成了三种新型表面活性剂样肽，并在体外成功表达。通过对表达宿主、表达载体和发酵条件的优化，最终得到最佳表达量。此外，这些表面活性剂样肽被发现具有良好的表面活性和低临界胶束浓度。更重要的是，它们具有pH响应性，可以在不同溶液中自组装形成纳米结构，这为未来新型纳米材料的生产提供了必要的研究价值。本研究首次通过生物方法构建和表达三种新型表面活性剂样肽，与化学方法相比，有效降低了生产成本；并且还发现它具有优异的表面活性剂性能。

更新日期：2022-03-29

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