Abstract

Objective: To explore the effect of music in pain perception and the molecular mechanism. Methods: Mice were separated to control, music, music + inhibitor, and saline group. Percentage pain reduction of mice in each group was determined. The contents of 5-HT, Glu, and NE in cerebrospinal fluid of mice in the four groups were determined by HPLC. FISH was used to analyze the distribution characteristics of GRIK5 in hippocampal tissues. The protein expressions of GRIK5, NMDA 1, and TRPC1 in the hippocampal tissues were determined via western blot. Results: Mice exposed to music showed a higher percentage of pain reduction compared to model animals, while injection of GRIK5 inhibitor weakened the effect of music. It increased the contents of 5-HT, Glu, and NE in mouse models of depression. The fluorescence signal in music group was significantly stronger than saline group. Music remarkably increased the expression of GRIK5, DLG4, NMDA 1, and TRPC1 proteins when compared with saline group. Conclusion: Music promoted the secretion of Glu. The glutaminergic pathway sensed Glu, subsequently activated GRIK5, DLG4, and NMDA 1 proteins to excite dopaminergic neurons, and finally promoted the production of 5-HT.

摘要

目的：探讨音乐对疼痛感知的影响及其分子机制。方法：将小鼠分为对照组、音乐组、音乐+抑制剂组和生理盐水组。测定每组小鼠的疼痛减轻百分比。采用高效液相色谱法测定四组小鼠脑脊液中5-HT、Glu、NE的含量。FISH用于分析GRIK5在海马组织中的分布特征。通过蛋白质印迹测定海马组织中 GRIK5、NMDA 1 和 TRPC1 的蛋白表达。结果：与模型动物相比，暴露于音乐的小鼠的疼痛减轻百分比更高，而注射 GRIK5 抑制剂则减弱了音乐的效果。它增加了抑郁症小鼠模型中 5-HT、Glu 和 NE 的含量。音乐组荧光信号明显强于生理盐水组。与生理盐水组相比，音乐显着增加了 GRIK5、DLG4、NMDA 1 和 TRPC1 蛋白的表达。结论：音乐促进Glu的分泌。谷氨酰胺能通路感知 Glu，随后激活 GRIK5、DLG4 和 NMDA 1 蛋白以激发多巴胺能神经元，最终促进 5-HT 的产生。